Mechanical stretch stimulates interleukin-8 production in endometrial stromal cells: Possible implications in endometrium-related events
Mechanical stretch stimulates interleukin-8 production in endometrial stromal cells: Possible implications in endometrium-related events
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DOI：
10.1210/jc.2004-1089
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发表时间：
2005-02-01
影响因子：
5.8
通讯作者：
Taketani, Y
中科院分区：
文献类型：
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作者：
Harada, M;Osuga, Y;Taketani, Y
子宫运动被认为在与子宫有关的各种事件中发挥作用。鉴于目前强调机械牵张的生物学意义的概念，我们推测子宫运动产生的机械牵张可能刺激子宫内膜细胞产生生化介质，并有助于炎症相关过程，如月经和子宫内膜异位症。为了解决机械拉伸在子宫内膜中可能产生的影响，将子宫内膜基质细胞（ESC）培养在柔性底培养板上，并使用计算机操作的细胞张力系统在无血清条件下以每分钟两个周期的速率施加周期性拉伸（25%伸长率）。ELISA法检测培养液中IL-8浓度，RT-PCR法检测ESC中IL-8 mRNA表达。周期性牵张可促进ESC分泌IL-8。IL-8分泌的增加被细胞外信号调节激酶1/2的抑制剂PD 98059抑制。孕酮也能抑制这种增加。另外，周期性牵张培养的ESC条件培养基刺激静止培养的ESC中IL-8 mRNA的表达。这些结果表明，子宫运动通过刺激子宫内膜中生化介质的产生对子宫内膜相关的生理和病理产生影响。
Uterine movement is suggested to play roles in various events related to the uterus. In view of the current concept underscoring the biological implications of mechanical stretch, we speculated that the mechanical stretch exerted by uterine movement might stimulate the production of biochemical mediators in endometrial cells and contribute to inflammation-associating processes, such as menstruation and endometriosis. To address the possible effects of mechanical stretch in the endometrium, endometrial stromal cells (ESC) were cultured on flexible-bottomed culture plates, and cyclic stretch (25% elongation) was applied in serum-free conditions at a rate of two cycles per minute using a computer-operated cell tension system. IL-8 concentrations in the conditioned medium were measured using ELISA, and IL-8 mRNA expression in ESC was measured by RT-PCR. Cyclic stretch increased the secretion of IL-8 from ESC. The increase in IL-8 secretion was inhibited by PD98059, an inhibitor of extracellular signal-regulated kinase 1/2. The increase was also inhibited by progesterone. In addition, the conditioned medium of ESC cultured with cyclic stretch stimulated the mRNA expression of IL-8 in ESC cultured under stationary conditions. These findings imply that uterine movement has an impact on endometrium-related physiology and pathology by stimulating the production of a biochemical mediator(s) in the endometrium.