[Embryo quality evaluation according to the speed of the first cleavage after conventional IVF].
[Embryo quality evaluation according to the speed of the first cleavage after conventional IVF].
复制标题
[根据常规IVF后首次卵裂速度评价胚胎质量]
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
M. Krskova
中科院分区：
文献类型：
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作者：
J. Biezinová;M. Svobodová;I. Oborna;H. Fingerová;J. Dosta;M. Krskova
目标
评估常规体外受精过程中获得的胚胎的形态参数。
设计
一项前瞻性研究。
设置
奥洛穆茨帕拉茨基大学医学院妇产科辅助生殖中心。
方法
在本研究中，对 2001 年至 2004 年期间传统 IVF 后发育的 549 个胚胎进行了评估。在授精后24-27小时的间隔内评估有丝分裂的开始。根据分裂速度将胚胎分为三组：早期卵裂（EC）胚胎，其中在评估时存在两个卵裂球；分解原核阶段（BDPN），其中原核已经消失；以及原核（PN）胚胎，其中两个原核仍然存在。在这些组中，在培养第二天评估破碎程度，并将胚胎分为四类：A - 具有规则的卵裂球，没有破碎，B - 具有不规则的卵裂球或破碎率低于30％，C - 破碎率30-50％，D - 破碎率高于50％。在培养的第二天和第三天评估进一步卵裂的速度和卵裂球的平均数量。统计分析在帕拉茨基大学计算机中心进行。采用独立样本的卡方检验和t检验。
结果
EC胚胎占45.5%，BDPN占33.5%，PN占21.0%。 EC 胚胎碎片较少 (p = 0.000)，在评估时具有更多卵裂球 (p = 0.000)，并且卵裂速度更快 (p = 0.000)。与 PN 组相比，EC 胚胎的卵裂速度更快 (p = 0.000)，但在培养第二天 EC 和 BDPN 组之间没有显着差异。第三天，EC 组和 BDPN 组之间也发现显着差异 (p = 0.000)。
结论
第一次细胞分裂的速度是 ET 胚胎选择的一个有用的附加标准。 EC 胚胎通常比 BPDN 和 PN 胚胎具有更好的形态和更多的卵裂球。
OBJECTIVE
To evaluate morphological parametres of embryos obtained in the process of conventional IVF.
DESIGN
A prospective study.
SETTING
Centre of Assisted Reproduction, Dept. of Obstetrics and Gynecology, Palacký University Medical School, Olomouc.
METHODS
In the present study 549 embryos developing after conventional IVF in the period of 2001-2004 were evaluated. The beginning of the mitotic cleavage was assessed within the interval of 24 - 27 hours after insemination. The embryos were divided into three groups according to the speed of their division as early cleavage (EC) embryos, where two blastomeres were present at the time of assessment, break down pronuclei stage (BDPN) where the pronuclei had already disappeared, and pronuclei (PN) embryos, where both pronuclei were still present. In these groups the degree of fragmentation was evaluated on day two of cultivation and embryos were divided into four categories as: A - with regular blastomeres, without fragmentation, B - with irregular blastomeres or with fragmentation below 30%, C - with fragmentation 30-50%, D - with fragmentation above 50%. The speed of further cleavage and average number of blastomeres were evaluated on days two and three of cultivation. Statistical analysis was preformed in the Palacký University Computer Centre. The chi-square test and t test for independent samples were used.
RESULTS
EC embryos were found in 45.5%, BDPN in 33.5% and PN in 21.0%. EC embryos were less fragmented (p = 0.000), had more blastomeres at the time of evaluation (p = 0.000) and their speed of cleavage was faster (p = 0.000). The cleavage of EC embryos was faster in comparisson with the PN group (p = 0.000), but there were no significant differences between the EC and BDPN groups on day two of cultivation. On day three significant difference was found also between the EC and BDPN groups (p = 0.000).
CONCLUSION
The speed of the first cell cleavage is a useful additional criterion for the embryo selection for ET. EC embryos usually have better morphology and more blastomeres that the BPDN and PN ones.