cDNA cloning and the response to overfeeding in the expression of stearoyl-CoA desaturase 1 gene in Landes goose
cDNA cloning and the response to overfeeding in the expression of stearoyl-CoA desaturase 1 gene in Landes goose
复制标题
朗德鹅硬脂酰辅酶A去饱和酶1基因的cDNA克隆及其对过度饲喂的反应
DOI：
10.1016/j.gene.2012.09.131
复制
发表时间：
2013-01-10
期刊：
影响因子：
3.5
通讯作者：
Gong, Daoqing
中科院分区：
文献类型：
--
作者：
Zhang, Rui;Zhu, Lihui;Gong, Daoqing
硬脂酰辅酶A去饱和酶1（SCD 1）是单不饱和脂肪酸生物合成中的限速酶。它已被克隆的几个物种：褐家鼠，小家鼠，智人和原鸡，但没有从雁。本研究旨在克隆朗德鹅SCD 1基因的cDNA序列，并研究过量饲养对SCD 1基因组织表达的影响。该基因全长3294 bp，开放阅读框1.083 bp，编码360个氨基酸，5 '/3'-UTR分别为74和2137 bp。采用定量真实的时间PCR（qPCR）检测过饲组和对照组心脏、肝脏、脾脏、肺、肾脏、砂囊、腺胃、肠、小腿、胸肌、下丘脑和脂肪组织（腹部脂肪）中SCD 1的表达。SCD 1 mRNA在鹅脂肪肝组织中高表达，过量饲养组肝脏和脂肪中SCD 1的表达量均比对照组高一倍以上。在过度饲养期间，肝脏和脂肪组织中SCD 1的表达在70 d后达到最高水平，但在79 d时下降。对照组在禁食24 h后，组织中SCD 1基因的表达水平急剧下降。然而，下丘脑中的SCD 1基因表达不受影响。本研究结果为研究SCD 1基因表达与朗德鹅脂肪肝形成的关系提供了理论依据。皇冠版权所有（c）2012由爱思唯尔B. V.出版保留所有权利。
Stearoyl-CoA desaturase 1 (SCD1) is a rate limiting enzyme in the biosynthesis of monounsaturated fatty acids. It has been cloned from several species: Rattus norvegicus, Mus musculus, Homo Sapiens and Gallus gallus, but not from Anser anser. This study was conducted to isolate the SCD1 cDNA sequence and investigate the effect of overfeeding on SCD1 gene tissue expression in Landes goose. The complete cDNA is 3294 bp in length, with an ORF of 1.083 bp encoding a predicted polypeptide of 360 amino acids and 5'/3'-UTR of 74 and 2137 bp, respectively. Quantitative real time PCR (qPCR) was used to examine SCD1 expression in heart, liver, spleen, lung, kidney, gizzard, glandular stomach, intestine, crureus, pectoral muscle, hypothalamus and adipose tissue (abdominal fat) in both the overfed and control group. SCD1 mRNA was highly expressed in goose fatty liver, and the expression levels of SCD1 in liver and fat of overfeeding group were more than double that of the control group. During the overfeeding period, SCD1 expression in liver and adipose tissue reached the highest level after 70 days, but declined at 79 days. In the control group, after fasting 24 h, the expression level of SCD1 gene in tissues declined sharply. However, SCD1 gene expression in hypothalamus was unaffected. The results of this study provide a theoretical basis to study the relationship between SCD1 gene expression and the formation of fatty liver of Landes goose in response to overfeeding. Crown Copyright (c) 2012 Published by Elsevier B.V. All rights reserved.