MUTATIONS AFFECTING IRON TRANSPORT IN ESCHERICHIA-COLI
MUTATIONS AFFECTING IRON TRANSPORT IN ESCHERICHIA-COLI
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DOI：
10.1128/jb.104.1.219-226.1970
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发表时间：
1970-01-01
影响因子：
3.2
通讯作者：
ROSENBERG, H
中科院分区：
文献类型：
--
作者：
COX, GB;GIBSON, F;ROSENBERG, H
大肠杆菌k -12突变体无法形成肠切蛋白依赖性铁转运系统的重要组成部分。该菌株携带了一个名为fep的基因突变，与两个与肠切素合成有关的基因enta和entd接近。菌株AN102携带fep -等位基因，可积累大量肠切素，并对柠檬酸钠产生生长反应。细胞色素1和总铁含量以及对55fe3 +摄取的测量表明肠切蛋白依赖的铁运输系统受损。对柠檬酸钠的生长响应与菌株AN102中诱导的依赖柠檬酸盐的铁转运系统的存在有关。
A mutant ofEscherichia coliK-12 unable to form an essential component of the enterochelin-dependent iron transport system has been isolated. This strain carries a mutation in a gene designatedfep, mapping close to two genes,entAandentD, concerned with enterochelin synthesis. Strain AN102, which carries thefep−allele, accumulates large quantities of enterochelin and gives a growth response to sodium citrate. The cytochromeb1and total iron content, and the measurement of the uptake of55Fe3+, indicate an impairment of the enterochelin-dependent iron transport system. The growth response to sodium citrate is related to the presence, in strain AN102, of an inducible citrate-dependent iron transport system.