Expression of pY397 FAK promotes the development of non-small cell lung cancer.
Expression of pY397 FAK promotes the development of non-small cell lung cancer.
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DOI：
10.3892/ol.2015.3992
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发表时间：
2016-02
期刊：
影响因子：
2.9
通讯作者：
Fu S
中科院分区：
文献类型：
--
作者：
Wang B;Qi X;Li D;Feng M;Meng X;Fu S
粘着斑激酶（FAK）的表达已被确定为与癌症的发展和转移。FAK在酪氨酸（Y）397（pY 397）处的自磷酸化在肿瘤细胞信号传导中起关键作用。然而，很少有研究评估pY 397 FAK在非小细胞肺癌（NSCLC）中的表达。在本研究中，pY 397 FAK在NSCLC中的表达进行了研究，使用免疫组化。比较各组标本之间的pY 397 FAK染色评分，并研究临床和病理特征之间的关联。Kaplan-Meier生存曲线分析pY 397 FAK表达与NSCLC患者预后的关系。本研究的结果显示，pY 397 FAK表达定位于肺细胞的细胞质，并且与相应的非肿瘤组织相比，pY 397 FAK在NSCLC组织以及相关的转移组织中过表达。而pY 397 FAK在原发灶和淋巴结转移灶中的表达无显著性差异。此外，pY 397 FAK染色评分与NSCLC患者的肿瘤大小、性别、分化程度、组织类型、淋巴结转移或生存率无关。这些结果表明，pY 397 FAK参与NSCLC的发展，但不是该疾病的预后标志物。
Focal adhesion kinase (FAK) expression has been identified as associated with cancer development and metastasis. Autophosphorylation of FAK at tyrosine (Y) 397 (pY397) performs a critical role in tumor cell signaling. However, few studies have evaluated the expression of pY397 FAK in non-small cell lung cancer (NSCLC). In the present study, pY397 FAK expression in NSCLC was investigated using immunohistochemistry. pY397 FAK staining scores were compared between various groups of specimens and the associations between clinical and pathological characteristics were investigated. A Kaplan-Meier survival curve was used to determine the association between pY397 FAK expression and the prognosis of NSCLC patients. The results of the present study revealed that pY397 FAK expression was localized to the cytoplasm of lung cells, and that pY397 FAK was overexpressed in NSCLC tissues, as well as associated metastatic tissues, when compared with the corresponding non-tumor tissues. However, no significant difference was identified between the pY397 FAK expression in primary lesions and lymph node metastases. Furthermore, pY397 FAK staining scores were not found to be associated with the tumor size, gender, degree of differentiation, histotypes, presence of lymph node metastases or survival rate of NSCLC patients. These results indicate that pY397 FAK is involved with the development of NSCLC, but is not a prognostic marker for the disease.