The day-to-day stability of the ruminal and fecal microbiota in lactating dairy cows
The day-to-day stability of the ruminal and fecal microbiota in lactating dairy cows
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泌乳奶牛瘤胃和粪便微生物群的日常稳定性
DOI：
10.1002/mbo3.990
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发表时间：
2020-03-16
期刊：
影响因子：
3.4
通讯作者：
Li, Shengli
中科院分区：
文献类型：
--
作者：
Huang, Shuai;Ji, Shoukun;Li, Shengli
在这项研究中，我们研究了之间的差异瘤胃液（DR）和粪便（DF）从5个泌乳奶牛连续三天使用16 S rRNA基因序列为基础的分析微生物群。结果表明，DR和DF的微生物群落之间存在显着差异。特别地，厚壁菌门和放线菌门的相对丰度在DR中显著低于DF（q < 0.001），而拟杆菌门的相对丰度在DF中显著高于DR（q < 0.001）。在DF中观察到双歧杆菌属、5- 7 N15、梭状芽孢杆菌属、Epulopiscium、SMB 53、Turicibacter、Dorea、Roseburia和Akkermansia的相对丰度显著更高，而普雷沃氏菌属、丁酸弧菌属、CF 231、RFN 20、使用功能预测程序PICRUSt的进一步分析表明，属于DRI 301和Succiniclasticum的序列在DR中被观察到。5- 7 N15、阿克曼氏菌、双歧杆菌、梭菌、多雷氏菌、浮鱼藻、罗斯拜瑞氏菌和Turicibacter与多糖合成代谢呈显著正相关; CF231、普氏菌、RFN 20和琥珀酸解脂菌与氨基酸、脂类、碳水化合物、其他氨基酸、辅因子和维生素代谢呈显著正相关。在DR或DF生态系统中，连续三天没有观察到显著差异，门和属水平的多样性或丰度没有显著差异，表明肠道微生物群的日常变化有限。
In this study, we examined differences between the microbiota of the ruminal fluid (DR) and feces (DF) from five lactating dairy cows over three consecutive days using 16S rRNA gene sequence-based analysis. Results showed significant differences between the microbial communities of the DR and DF. In particular, the relative abundance of the phyla Firmicutes and Actinobacteria was significantly lower (q < 0.001) in DR compared with DF, while the relative abundance of Bacteroidetes was significantly higher in DF than that of DR (q < 0.001). A significantly higher relative abundance of the genera Bifidobacterium, 5-7N15, Clostridium, Epulopiscium, SMB53, Turicibacter, Dorea, Roseburia, and Akkermansia was observed in the DF, while a higher relative abundance of the genera Prevotella, Butyrivibrio, CF231, RFN20, and Succiniclasticum was observed in the DR. A further analysis using the functional prediction program PICRUSt showed that sequences belonging to the 5-7N15, Akkermansia, Bifidobacterium, Clostridium, Dorea, Epulopiscium, Roseburia, and Turicibacter were significantly and positively correlated with glycan biosynthesis and metabolism, while CF231, Prevotella, RFN20, and Succiniclasticum were significantly and positively correlated with amino acid, lipid, carbohydrate, other amino acid, cofactors, and vitamins metabolism. No significant differences were observed across the three consecutive days in either the DR or DF ecosystems, with no significant differences in the diversity or abundance at the phylum and genus levels suggested that there is a limited day-to-day variability in the gut microbiota.