Differences in Exhaled Gas Profiles Between Patients with Type 2 Diabetes and Healthy Controls
Differences in Exhaled Gas Profiles Between Patients with Type 2 Diabetes and Healthy Controls
复制标题
DOI：
10.1089/dia.2009.0181
复制
发表时间：
2010-06-01
影响因子：
5.4
通讯作者：
Paretzke, H. G.
中科院分区：
文献类型：
--
作者：
Greiter, M. B.;Keck, L.;Paretzke, H. G.
目的：分析技术的最新进展允许检测人体呼出空气中的数百种挥发性有机化合物（VOC），其中许多反映了未识别的内源性途径。本研究进行，以确定是否呼吸气体分析，质子转移反应-质谱（PTRMS）可以作为一种非侵入性的方法来区分2型糖尿病患者和健康controls.Methods：呼吸和室内空气样本进行了测量，从21例胰岛素治疗的2型糖尿病患者和26名健康对照。在20-200原子质量单位的质量范围内的挥发性有机化合物进行了分析，使用PTR-MS。结果：我们确定了8个质量特征的内源性挥发性有机化合物，显示出显着差异的2型糖尿病患者和健康对照组的气体配置文件。使用这些挥发性有机化合物的线性判别分析，灵敏度和特异性被发现是90%和92%，respectives.Conclusions：这些结果表明，它是可能的，从健康对照组的2型糖尿病患者呼出内源性挥发性有机化合物的多变量分析。这是朝着开发使用高危人群呼吸气体的非侵入性测试迈出的第一步，并使其成为对高危人群进行大规模测试的有吸引力的选择。然而，呼出挥发物作为代谢标志物的建立需要额外的验证性研究。
Aims: Recent advances in analytical technology allow the detection of several hundred volatile organic compounds (VOCs) in human exhaled air, many of which reflect unidentified endogenous pathways. This study was performed to determine whether a breath gas analysis using proton transfer reaction-mass spectrometry (PTRMS) could serve as a noninvasive method to distinguish between patients with type 2 diabetes mellitus and healthy controls.Methods: Breath and room air samples were measured from 21 patients with insulin-treated type 2 diabetes and 26 healthy controls. VOCs in the mass range of 20-200 atomic mass units were analyzed using PTR-MS.Results: We identified eight masses characteristic of endogenous VOCs that showed significant differences in the gas profiles of patients with type 2 diabetes and healthy control subjects. Using these VOCs for linear discriminant analysis, the sensitivity and specificity were found to be 90% and 92%, respectively.Conclusions: These results suggest that it is possible to separate patients with diabetes mellitus type 2 from healthy controls by multivariate analysis of exhaled endogenous VOCs. This is a first step towards the development of a noninvasive test using breath gas of at-risk persons and making it an attractive option for large-scale testing of at-risk populations. However, the establishment of exhaled volatiles as metabolic markers requires additional confirmatory investigations.