Antibody W7C5 defines a CD109 epitope expressed on CD34+ and CD34- hematopoietic and mesenchymal stem cell subsets
Antibody W7C5 defines a CD109 epitope expressed on CD34+ and CD34- hematopoietic and mesenchymal stem cell subsets
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DOI：
10.1111/j.1749-6632.2003.tb03250.x
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发表时间：
2003-01-01
期刊：
影响因子：
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通讯作者：
Bühring, HJ
中科院分区：
文献类型：
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作者：
Giesert, C;Marxer, A;Bühring, HJ
单克隆抗体W7C5识别的细胞表面抗原在人CD34（+）和CD34（-）骨髓干细胞群中表达水平较低，但在胎儿肝脏CD34（+）细胞中表达水平较高。为了鉴定识别抗原，我们对纯化的W7C5抗原进行了部分氨基酸序列和MALDI分析。分析显示，MoAb W7C5检测到CD109，这是一种最近克隆的糖磷脂酰肌醇连接糖蛋白。通过对转染人CD109 cDNA的CHO细胞的选择性识别，进一步证实了MoAb W7C5的特异性。
The cell surface antigen recognized by monoclonal antibody W7C5 is expressed at low levels on human CD34(+) and CD34(-) bone marrow stem cell populations but at high levels on fetal liver CD34(+) cells. To identify the recognized antigen, we performed partial amino acid sequence and MALDI analysis of purified W7C5 antigens. The analyses revealed that MoAb W7C5 detects CD109, a recently cloned glycophosphatidylinositol-linked glycoprotein. The specificity of MoAb W7C5 was further confirmed by the selective recognition of CHO cells transfected with human CD109 cDNA.