RecA expression in response to solar UVR in the marine bacterium Vibrio natriegens
RecA expression in response to solar UVR in the marine bacterium Vibrio natriegens
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DOI：
10.1007/s00248-001-1009-5
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发表时间：
2001-11-01
影响因子：
3.6
通讯作者：
Miller, RV
中科院分区：
文献类型：
--
作者：
Booth, MG;Jeffrey, WH;Miller, RV
太阳紫外线辐射可能对海洋和河口社区造成日常压力，因为细胞受损并修复这种损害。地球平流层臭氧层的减少提高了人们对紫外线辐射可能对环境产生的潜在影响的认识，包括海洋细菌如何对太阳辐射的变化作出反应。我们研究了使用细菌RecA蛋白作为细菌修复太阳紫外线照射引起的DNA损伤的潜力的指标，使用海洋细菌弧菌natriegens作为模型。RecA普遍存在于细菌中，并且是所谓的暗修复系统的调节蛋白，所述暗修复系统包括切除修复、复制后重组修复和诱变或SOS修复。太阳UVB和UVA都降低了海水微宇宙中产钠弧菌的活力。暴露于未经过滤的太阳辐射或UVB被阻挡的辐射后，存活率下降到1%以下，而UVA和UVB被过滤的可见光对存活率没有影响。使用RecA特异性抗体进行检测，RecA蛋白诱导太阳辐射在一个昼夜模式的海洋缩影在墨西哥湾进行。每天黄昏时观察到诱导峰值。虽然RecA表达与UVB诱导的环丁基嘧啶二聚体的形成相关，但较长波长的UVA辐射也诱导recA基因表达。我们的研究结果表明，RecA调节，光独立的修复是海洋细菌的生存暴露于太阳紫外线辐射的能力的一个重要组成部分，RecA的表达是一个有用的监测暴露于太阳紫外线辐射后的细菌修复。
Solar ultraviolet radiation may produce daily stress on marine and estuarine communities as cells are damaged and repair that damage. Reduction in the earth's stratospheric ozone layer has increased awareness of the potential effects that ultraviolet radiation may have in the environment, including how marine bacteria respond to changes in solar radiation. We examined the use of the bacterial RecA protein as an indicator of the potential of bacteria to repair DNA damage caused by solar UV irradiation using the marine bacterium Vibrio natriegens as a model. RecA is universally present in bacteria and is a regulator protein for the so-called Dark Repair Systems, which include excision repair, postreplication recombinational repair, and mutagenic or SOS repair. Solar UVB and UVA both reduced V. natriegens viability in seawater microcosms. After exposure to unfiltered solar radiation or radiation in which UVB was blocked, survival dropped below 1%, whereas visible light from which UVA and UVB had been filtered had no effect on survival. Using a RecA-specific antibody for detection, RecA protein was induced by solar radiation in a diel pattern in marine microcosms conducted in the Gulf of Mexico. Peak induction was observed at dusk each day. Although RecA expression was correlated with the formation of UVB-induced cyclobutyl pyrimidine dimers, longer wavelength UVA radiation also induced recA gene expression. Our results demonstrate that RecA-regulated, light-independent repair is an important component in the ability of marine bacteria to survive exposure to solar ultraviolet radiation and that RecA expression is a useful monitor of bacterial repair after exposure to solar UVR.