PROTEIN A FROM STAPHYLOCOCCUS-AUREUS - ITS ISOLATION BY AFFINITY CHROMATOGRAPHY AND ITS USE AS AN IMMUNOSORBENT FOR ISOLATION OF IMMUNOGLOBULINS
PROTEIN A FROM STAPHYLOCOCCUS-AUREUS - ITS ISOLATION BY AFFINITY CHROMATOGRAPHY AND ITS USE AS AN IMMUNOSORBENT FOR ISOLATION OF IMMUNOGLOBULINS
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DOI：
10.1016/0014-5793(72)80680-x
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发表时间：
1972-01-01
期刊：
影响因子：
3.5
通讯作者：
SJOQUIST, J
中科院分区：
文献类型：
--
作者：
HJELM, H;HJELM, K;SJOQUIST, J
来自金黄色葡萄球菌的蛋白A与细胞壁的肽聚糖部分共价连接[1，21]。它可以通过溶葡萄球菌酶消化从细胞中释放出来，这个过程是从细菌中分离蛋白质的第一步[1]。蛋白质的进一步纯化涉及酸和盐沉淀、离子交换色谱和凝胶过滤。通过该技术的蛋白A的产量约为100%。由于蛋白A与IgG的Fc部分反应[3，41]，因此偶联到琼脂糖凝胶的来自IgG的非免疫IgC或Fc可用作分离蛋白的免疫吸附剂。相反，与琼脂糖凝胶结合的蛋白A可以用作免疫吸附剂，用于从几个物种中分离IgG以及用于分离Fc级分。本文介绍了从S.金黄色葡萄球菌和人血清IgG的亲和层析。此外，将讨论结合到固体支持物上的蛋白A作为免疫吸附剂的几种用途。
Protein A from Staphylococcus aureus is covalently linked to the peptidoglycan part of the cell wall [1, 21. It can be released from the cells by digestion with lysostaphin, and this procedure is the initial step in the isolation of the protein from the bacteria [l]. Further purification of the protein has involved acid and salt precipitation, ion exchange chromatography and gel filtration. The yield of protein A by this technique is approx. 5% of the total amount of the protein in the cell [1].Since protein A reacts with the Fc-part of IgG [3, 41, non-immune IgC or Fc from IgG coupled to Sepharose can be used as an immunosorbent for isolation of the protein. Conversely, protein A bound to Sepharose can be used as an immunosorbent for isolation of IgG from several species as well as for the isolation of the Fc-fraction. This article describes the isolation of protein A from S. aureus and of IgG from human serum by affinity chromatography. In addition, several uses for protein A bound to a solid support as an immunosorbknt will be discussed.