Subtraction by addition: domesticated transposases in programmed DNA elimination.
Subtraction by addition: domesticated transposases in programmed DNA elimination.
复制标题
加减法：程序化 DNA 消除中的驯化转座酶。
DOI：
10.1101/gad.1864609
复制
发表时间：
2009
影响因子：
10.5
通讯作者：
Chalker,DouglasL
中科院分区：
文献类型：
--
作者：
Motl,JasonA;Chalker,DouglasL
四脲草履虫（Paramecium tetrauredae）的体细胞大核必须消除基因组中约60，000个短序列，才能产生不间断的编码序列。在这一期的《基因与发育》中，Baudry和他的同事们（pp. 2478-2483）鉴定了切除这些非编码序列的蛋白质：一种驯化的piggyBacTM转座酶，该转座酶已被改造以去除可能是转座子插入的残余物。这项新的研究揭示了在小RNA定向沉默机制中添加转座酶如何指导主要基因组重组。
The ciliateParamecium tetraureliamust eliminate ∼60,000 short sequences from its genome to generate uninterrupted coding sequences in its somatic macronucleus. In this issue ofGenes & Development, Baudry and colleagues (pp. 2478–2483) identify the protein that excises these noncoding sequences: a domesticatedpiggyBactransposase that has been adapted to remove what are likely the remnants of transposon insertions. This new study reveals how addition of a transposase to small RNA-directed silencing machinery can guide major genome reorganization.