Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method.
Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method.
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DOI：
10.1101/pdb.prot100479
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发表时间：
2019-02-01
影响因子：
--
通讯作者：
Sambrook, Joseph
中科院分区：
文献类型：
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作者：
Green, Michael R;Sambrook, Joseph
从聚丙烯酰胺凝胶中回收DNA片段的标准方法是“压碎和浸泡”技术。洗脱的DNA通常不含抑制酶或对转染或显微注射的细胞有毒的污染物。该方法需要时间，但劳动力很少，
The standard method to recover fragments of DNA from polyacrylamide gels is the "crush and soak" technique. The eluted DNA is generally free of contaminants that inhibit enzymes or that are toxic to transfected or microinjected cells. The method requires time but little labor and results in recovery of