Axonal netrin-Gs transneuronally determine lamina-specific subdendritic segments
Axonal netrin-Gs transneuronally determine lamina-specific subdendritic segments
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DOI：
10.1073/pnas.0706919104
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发表时间：
2007-09-11
影响因子：
11.1
通讯作者：
Itohara, Shigeyoshi
中科院分区：
文献类型：
--
作者：
Nishimura-Akiyoshi, Sachiko;Niimi, Kimie;Itohara, Shigeyoshi
来自不同神经元组的轴突以明显分离和分层的模式与靶神经元的树突树接触，从而形成用于在脊椎动物中枢神经系统中处理多个信息输入的功能单元。树突是否以及如何获得对应于每个通路的层特异性特性尚不清楚。在这里，我们表明，脊椎动物特定的膜锚定的成员的β-6/ netrin家族，netrin-G1和-G2，组织层/通路特异性分化的树突。Netrin-G1和Netrin-G2分布于不同途径的轴突上，分别与受体NGL-1和NGL-2特异性相互作用。在海马、顶叶皮质和梨状皮质中，NGL-1集中在对应于netrin-G1阳性轴突的层特异性终止的树突段中，并且NGL-2集中在对应于netrin-G2阳性轴突的终止的不同树突段中。在netrin-G1-和-G2-缺陷小鼠中，其中轴突寻径是正常的，NGL-1和-2的节段分布被选择性地破坏，并且单个受体沿着树突扩散。这些研究结果表明，netrin-Gs及其特异性受体的跨神经元的相互作用提供了一个轴突神经支配依赖的机制突触后膜组织的分子基础，并提供洞察树突内的层状结构的形成。
Axons from a distinct group of neurons make contact with dendritic trees of target neurons in clearly segregated and laminated patterns, thereby forming functional units for processing multiple inputs of information in the vertebrate central nervous system. Whether and how dendrites acquire lamina-specific properties corresponding to each pathway is not known. We show here that vertebrate-specific membrane-anchored members of the UNC-6/ netrin family, netrin-G1 and -G2, organize the lamina/pathway-specific differentiation of dendrites. Netrin-G1 and -G2 distribute on axons of different pathways and specifically interact with receptors NGL-1 and -2, respectively. In the hippocampus, parietal cortex, and piriform cortex, NGL-1 is concentrated in the dendritic segments corresponding to the lamina-specific termination of netrin-G 1 -positive axons, and NGL-2 is concentrated in distinct dendritic segments corresponding to the termination of netrin-G2-positive axons. In netrin-G1- and -G2-deficient mice, in which axonal path-finding is normal, the segmental distribution of NGL-1 and -2 is selectively disrupted, and the individual receptors are diffused along the dendrites. These findings indicate that trans-neuronal interactions of netrin-Gs and their specific receptors provide a molecular basis for the axonal innervation-dependent mechanism of postsynaptic membrane organization, and provide insight into the formation of the laminar structure within the dendrites.