Dynamic changes in histone modification are associated with upregulation of Hsf and rRNA genes during heat stress in maize seedlings
Dynamic changes in histone modification are associated with upregulation of Hsf and rRNA genes during heat stress in maize seedlings
复制标题
组蛋白修饰的动态变化与玉米幼苗热应激期间 Hsf 和 rRNA 基因的上调有关
DOI：
10.1007/s00709-019-01364-4
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发表时间：
2019-09-01
期刊：
影响因子：
2.9
通讯作者：
Li, Lijia
中科院分区：
文献类型：
--
作者：
Hou, Haoli;Zhao, Lin;Li, Lijia
组蛋白修饰在植物对非生物胁迫的响应中起着重要的作用。然而，很少有科学的研究参与组蛋白修饰的动态变化在玉米热胁迫反应。本研究旨在分析热胁迫下玉米幼苗生理生化变化的表观遗传调控机制。结果表明，在高温下，质膜H ~+-ATPase的电解质渗漏和水解活性增加，色素含量和活性氧（ROS）含量增加。此外，在热应激期间观察到核糖体DNA（rDNA）染色质的解凝聚和rRNA基因表达的同时增加，伴随着组蛋白H3 K4 me 2和H3 K9 ac水平的全基因组增加。此外，染色质免疫沉淀（ChIP）分析显示，H3 K4 me 2和H3 K9 ac水平的改变发生在启动子区域，这被发现与热应激因子（Hsf）和rRNA基因的上调有关。总之，短期热胁迫诱导动态组蛋白的变化，这与HSF和rRNA基因转录，伴随着细胞膜的扰动和增加的ROS在玉米幼苗驯化过程中。
Histone modification plays a significant role in plant responses to abiotic stress. However, there are little scientific studies available on the involvement of dynamic changes in histone modification in the heat stress response in maize. The present investigation was aimed to analyze the epigenetic mechanisms involved in regulating the physiological and biochemical alterations in maize seedlings under heat stress. Our results and observations indicated an increase in electrolyte leakage and hydrolytic activity of the plasma membrane H+-ATPase as well as the high pigment content and reactive oxygen species (ROS) content under high temperature. Furthermore, decondensation of ribosomal DNA (rDNA) chromatin and a simultaneous increase in rRNA gene expression were observed during heat stress, accompanied by a genome-wide increase in the levels of histone H3K4me2 and H3K9ac. Additionally, chromatin immunoprecipitation (ChIP) analysis revealed that alterations in H3K4me2 and H3K9ac levels occurred in promoter regions, which were found to be associated with the upregulation of heat stress factor (Hsf) and rRNA genes. In conclusion, short-term heat stress induces dynamic histone alterations which are associated with Hsf and rRNA gene transcription, accompanied by perturbations of cell membranes and an increase in ROS during acclimation in maize seedlings.