Di-2-ethylhexyl phthalate (DEHP) induces apoptosis and autophagy of mouse GC-1 spg cells
Di-2-ethylhexyl phthalate (DEHP) induces apoptosis and autophagy of mouse GC-1 spg cells
复制标题
邻苯二甲酸二-2-乙基己酯 (DEHP) 诱导小鼠 GC-1 spg 细胞凋亡和自噬
DOI：
10.1002/tox.22866
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发表时间：
2019-11-01
影响因子：
4.5
通讯作者：
Chen, Jiaxiang
中科院分区：
文献类型：
--
作者：
Gan, Yu;Yang, Dan;Chen, Jiaxiang
邻苯二甲酸二（2-乙基己基）酯（DEHP）是一种广泛应用的增塑剂，可引起睾丸毒性，但其作用机制尚不清楚。在这项研究中，DEHP暴露显着抑制细胞活力和诱导小鼠GC-1 spg细胞凋亡。此外，DEHP显著增加了自噬蛋白LC 3-II、Beclin 1和Atg 5的水平，以及LC 3-II/LC 3-I的比例。透射电子显微镜（TEM）进一步证实DEHP诱导小鼠GC-1 spg细胞自噬。DEHP也显示出诱导氧化应激;而NAC抑制氧化应激可以增加细胞活力并抑制DEHP诱导的细胞凋亡和自噬。这些结果表明，DEHP通过氧化应激诱导小鼠GC-1 spg细胞凋亡和自噬。自噬抑制剂3-MA可挽救DEHP诱导的细胞凋亡。综上所述，DEHP通过氧化应激诱导小鼠GC-1 spg细胞凋亡和自噬，自噬可能对DEHP诱导的细胞凋亡产生细胞毒性作用。
As a widely used plasticizer in industry, di-2-ethylhexylphthalate (DEHP) can cause testicular toxicity, yet little is known about the potential mechanism. In this study, DEHP exposure dramatically inhibited cellviability and induced apoptosis of mouse GC-1 spg cells. Furthermore, DEHP significantly increased the levels of autophagy proteins LC3-II, Beclin1 and Atg5, as well as the ratio ofLC3-II/LC3-I. Transmission electron microscopy (TEM) further confirmed that DEHP induced autophagy of mouse GC-1 spg cells. DEHP was also shown to induceoxidative stress; while inhibition of oxidative stress with NAC could increase cell viability and inhibit DEHP-induced apoptosis and autophagy. These results suggested that DEHP induced apoptosis and autophagy of mouse GC-1 spg cells via oxidative stress. 3-MA, an inhibitor of autophagy, could rescue DEHP-induced apoptosis. In summary, DEHP induced apoptosis and autophagy of mouse GC-1 spg cells via oxidative stress, and autophagy might exert a cytotoxic effect on DEHP-induced apoptosis.