High-sensitivity hyperspectral vibrational imaging of heart tissues by mid-infrared photothermal microscopy
High-sensitivity hyperspectral vibrational imaging of heart tissues by mid-infrared photothermal microscopy
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DOI：
10.1007/s44211-022-00182-8
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发表时间：
2022-09-07
影响因子：
1.6
通讯作者：
Tanaka, Takuo
中科院分区：
文献类型：
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作者：
Kato, Ryo;Yano, Taka-aki;Tanaka, Takuo
可视化生物组织中化学成分的空间分布对于研究基本生物过程和疾病起源具有重要意义。拉曼显微镜是一种无标记的振动成像技术，已被用于组织的化学表征。然而，拉曼光谱学的低灵敏度通常需要长的拉曼测量采集时间或高激光功率或两者，这妨碍了以非破坏性方式研究大面积组织。在这项工作中，我们展示了使用中红外光热（MIP）显微镜的心脏组织的化学成像，同时实现了得益于分子的IR吸收的高灵敏度和低至几微米的高空间分辨率。我们成功地可视化了不同生物分子的分布，包括蛋白质、含磷酸盐的蛋白质和脂质/碳水化合物/氨基酸。此外，我们实验比较MIP显微镜与拉曼显微镜，以评估灵敏度和光损伤的组织。我们证明，MIP显微镜是一种高灵敏度的技术，用于获得分子的振动信息在一个广泛的指纹区域，从而它可以用于生物和诊断应用，如活组织成像。
Visualizing the spatial distribution of chemical compositions in biological tissues is of great importance to study fundamental biological processes and origin of diseases. Raman microscopy, one of the label-free vibrational imaging techniques, has been employed for chemical characterization of tissues. However, the low sensitivity of Raman spectroscopy often requires a long acquisition time of Raman measurement or a high laser power, or both, which prevents one from investigating large-area tissues in a nondestructive manner. In this work, we demonstrated chemical imaging of heart tissues using mid-infrared photothermal (MIP) microscopy that simultaneously achieves the high sensitivity benefited from IR absorption of molecules and the high spatial resolution down to a few micrometers. We successfully visualized the distributions of different biomolecules, including proteins, phosphate-including proteins, and lipids/carbohydrates/amino acids. Further, we experimentally compared MIP microscopy with Raman microscopy to evaluate the sensitivity and photodamage to tissues. We proved that MIP microscopy is a highly sensitive technique for obtaining vibrational information of molecules in a broad fingerprint region, thereby it could be employed for biological and diagnostic applications, such as live-tissue imaging.