Morphology, phylogenetic position, and ecophysiology of Alexandrium ostenfeldii (Dinophyceae) from the Bohai Sea, China
Morphology, phylogenetic position, and ecophysiology of Alexandrium ostenfeldii (Dinophyceae) from the Bohai Sea, China
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DOI：
10.1111/j.1759-6831.2011.00160.x
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发表时间：
2011-11-01
影响因子：
3.7
通讯作者：
Gu, Haifeng
中科院分区：
文献类型：
--
作者：
Gu, Haifeng
奥氏亚历山大藻是一种具有潜在毒性的甲藻，常发生在高纬度沿海地区。在这里，我们报告的存在A。中国渤海海域的ostenfeldii属首次报道。A. ostenfeldii的特征是位于右前侧的第一顶板狭窄，腹孔大。部分大亚基序列比较显示，中国株与芬兰株仅在3个位置上不同，与A.西班牙的peruvianum在五个位置。最大简约分析表明，A. ostenfeldii和A.来自西班牙的peruvianum聚集在一起。它们是与A. ostenfeldii来自新西兰，欧洲和北美。在文化中，生长没有发生在温度低于9摄氏度，并发生在7和27 psu之间的盐度。新形成的包囊在20摄氏度下需要10-20天才能成熟。较低的温度延迟发芽，但在12至24 ℃的温度下发芽率超过90%。孵育1个月后，在低于9 ℃下未发生发芽。A. ostenfeldii既不产生螺内酯，也不产生麻痹性贝类毒素。
Alexandrium ostenfeldii is a potentially toxic dinoflagellate that often occurs in coastal areas at high latitudes. Here we report the presence of A. ostenfeldii in the Bohai Sea, China, for the first time. The vegetative cells of A. ostenfeldii are characterized by a narrow first apical plate and a large ventral pore located on the anterior right side. Partial large subunit sequence comparison revealed that the Chinese strain differs from the Finnish strains at only three positions, and from A. peruvianum of Spain at five positions. Maximum parsimony analysis revealed that A. ostenfeldii from China and Finland and A. peruvianum from Spain grouped together. They were the nearest sister group to a clade with A. ostenfeldii from New Zealand, Europe, and North America. In culture, growth did not occur at temperatures below 9 degrees C and occurred at salinities between 7 and 27 psu. It took 10-20 days for newly formed cysts to mature at 20 degrees C. Lower temperature delayed germination, but the germination rate exceeded 90% at temperatures from 12 to 24 degrees C. No germination occurred below 9 degrees C after 1 month of incubation. The Chinese strain of A. ostenfeldii produced neither spirolides nor paralytic shellfish poisoning toxins.