A New Formulation of Probiotics Attenuates Calcipotriol-Induced Dermatitis by Inducing Regulatory Dendritic Cells.
A New Formulation of Probiotics Attenuates Calcipotriol-Induced Dermatitis by Inducing Regulatory Dendritic Cells.
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DOI：
10.3389/fimmu.2021.775018
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发表时间：
2021
影响因子：
7.3
通讯作者：
Yao X
中科院分区：
文献类型：
--
作者：
Xu B;Ling S;Xu X;Liu X;Wang A;Zhou Y;Luo Y;Li W;Yao X
特应性皮炎（AD）是一种复发性慢性炎症性皮肤病，影响高达30%的儿童人口，免疫调节治疗，可以改变疾病的过程是迫切需要的。益生菌已被证明对AD有治疗作用，并可能调节疾病过程。然而，益生菌对AD的疗效在不同的研究中并不一致，这主要是由于益生菌的作用机制不明确以及所使用的菌种和（或）菌株不同。本研究设计了5株益生菌混合物（命名为IW5），并分析了IW5对卡泊三醇（MC903）诱导的AD样皮炎的作用及其机制。我们发现IW5显著减轻了MC903诱导的AD小鼠的皮肤炎症。施用IW5诱导肠系膜淋巴结中调节性T细胞和调节性树突状细胞（DCDC）的产生增加。我们还发现，在给予IW5后，患有MC903诱导的皮炎的小鼠中肠道微生物群的多样性增加，并且肠道中丁酸盐的水平升高。在细胞培养中，丁酸盐诱导DCreg的产生。我们的研究揭示了新设计的益生菌混合物的治疗效果，并揭示了可能的机制，为未来的临床研究提供了基础。
Atopic dermatitis (AD) is a recurrent chronic inflammatory skin disease affecting up to 30% of the children population, and immuno-regulatory therapy that could modify the course of disease is urgently needed. Probiotics have demonstrated therapeutic effects on AD and could potentially regulate the disease process. However, the efficacy of probiotics for AD is inconsistent among different studies, which is mainly due to the elusive mechanism and different species and (or) strains used. In this study, we designed a mixture of five strains of probiotics (named IW5) and analyzed the effect and mechanism of IW5 on calcipotriol (MC903)-induced AD-like dermatitis. We found that IW5 significantly alleviated skin inflammation of the MC903-induced AD in mice. Administration with IW5 induced increased production of regulatory T cells and regulatory dendritic cells (DCregs) in the mesenteric lymph nodes. We also found that the diversity of the gut microbiota in the mice with MC903-induced dermatitis was increased after IW5 administration, and the level of butyrate in the gut was elevated. In cell culture, butyrate induced the production of DCregs. Our study revealed the therapeutic effects of a newly designed probiotics mixture and uncovered a possible mechanism, providing a foundation for future clinical studies.