VEGF and VEGF-C: Specific induction of angiogenesis and lymphangiogenesis in the differentiated avian chorioallantoic membrane
VEGF and VEGF-C: Specific induction of angiogenesis and lymphangiogenesis in the differentiated avian chorioallantoic membrane
复制标题
DOI：
10.1006/dbio.1997.8639
复制
发表时间：
1997-08-01
影响因子：
2.7
通讯作者：
Wilting, J
中科院分区：
文献类型：
--
作者：
Oh, SJ;Jeltsch, MM;Wilting, J
内皮生长因子的淋巴管生成能力迄今尚未研究。这部分是由于缺乏体内淋巴管生成试验。用美世树脂显微注射、半切片和半切片、纤维连接蛋白和α-平滑肌肌动蛋白的免疫组织化学检测以及VEGFR-2和VEGFR-3探针的原位杂交等方法，研究了鸡胚绒毛尿囊膜（CAM）的分化特性。小动脉和动脉伴随着一对相互连接的分支，并在较大的动脉周围形成丛。静脉也与淋巴管有关，特别是被淋巴丛包围的大静脉。血管内皮细胞的特征是内皮层极薄，有孔，无基底层。细胞外基质的斑块可用抗纤连蛋白的抗体染色。分化CAM的淋巴管内皮细胞显示这种细胞类型的超微结构特征。CAM造影剂不具有介质。相反，脐柄的淋巴干被单层但不连续的平滑肌细胞覆盖，CAM趋化因子表达VEGFR-2和VEGFR-3。这两个规则的模式和典型的结构，这些淋巴管生成因子表明，CAM是一个合适的网站，研究在体内的潜在淋巴管生成因子的影响。我们已经研究了VEGF同二聚体和异二聚体、VEGF/PlGF异二聚体以及PlGF和VEGF-C同二聚体对第13天CAM的影响。所有含有至少一条VEGF链的生长因子都是血管生成性的，但不诱导淋巴管生成。PlGF-1和PlGF-2既不是血管生成的也不是淋巴管生成的。VEGF-C是第一个淋巴管生成因子，似乎对淋巴管内皮细胞具有高度的化学吸引力。它诱导淋巴管内皮细胞的增殖和直接位于绒毛膜上皮下方的新淋巴窦的发育。我们的研究表明VEGF和VEGF-C分别是特异性的血管生成和淋巴管生成生长因子。(C)北京：科学出版社.
The lymphangiogenic potency of endothelial growth factors has not been studied to date. This is partially due to the lack of in vivo lymphangiogenesis assays. We have studied the lymphatics of differentiated avian chorioallantoic membrane (CAM) using microinjection of Mercer resin, semi- and ultrathin sectioning, immunohistochemical detection of fibronectin and alpha-smooth muscle actin, and in situ hybridization with VEGFR-2 and VEGFR-3 probes, CAM is drained by lymphatic vessels which are arranged in a regular pattern. Arterioles and arteries are accompanied by a pair of interconnected lymphatics and form a plexus around bigger arteries. Veins are also associated with lymphatics, particularly larger veins, which are surrounded by a lymphatic plexus. The lymphatics are characterized by an extremely thin endothelial lining, pores, and the absence of a basal lamina, Patches of the extracellular matrix can be stained with an antibody against fibronectin. Lymphatic endothelial cells of differentiated CAM show ultrastructural features of this cell type. CAM lymphatics do not possess mediae. In contrast, the lymphatic trunks of the umbilical stalk are invested by a single but discontinuous layer of smooth muscle cells, CAM lymphatics express VEGFR-2 and VEGFR-3. Both the regular pattern and the typical structure of these lymphatics suggest that CAM is a suitable site to study the in vivo effects of potential lymphangiogenic factors. We have studied the effects of VEGF homo- and heterodimers, VEGF/PlGF heterodimers, and PlGF and VEGF-C homodimers on Day 13 CAM. All the growth factors containing at least one VEGF chain are angiogenic but do not induce lymphangiogenesis. PlGF-1 and PlGF-2 are neither angiogenic nor lymphangiogenic. VEGF-C is the first lymphangiogenic factor and seems to be highly chemoattractive for lymphatic endothelial cells. It induces proliferation of lymphatic endothelial cells and development of new lymphatic sinuses which are directed immediately beneath the chorionic epithelium. Our studies show that VEGF and VEGF-C are specific angiogenic and lymphangiogenic growth factors, respectively. (C) 1997 Academic Press.