Recombination in circulating Human enterovirus B:: independent evolution of structural and non-structural genome regions
Recombination in circulating Human enterovirus B:: independent evolution of structural and non-structural genome regions
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DOI：
10.1099/vir.0.81264-0
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发表时间：
2005-12-01
影响因子：
3.8
通讯作者：
Ilonen, J
中科院分区：
文献类型：
--
作者：
Lukashev, AN;Lashkevich, VA;Ilonen, J
测定了1998 ~ 2002年从前苏联分离的8株人肠道病毒B（HEV-B）的核苷酸全序列，它们分别代表E6、E7、E11、CVB 3和CVB 5五种血清型。所有毒株均为嵌合重组株，只有VP 2-VP 3-VP 1基因组区域与相应的原型HEV-B株相似。在所研究的八个毒株中的七个中，2C-3D基因组区域与原型E30、EV 74和EV 75毒株最相似，而剩余的毒株在非结构蛋白基因组区域中与原型El和E9毒株最相似。大多数病毒在基因组的这一部分还带有额外重组事件的标记。在5'非翻译区，所有毒株与原型E9比与其他肠道病毒更相似。在大多数情况下，重组映射到VP 4和2ABC基因组区域。这与这些基因组区域的系统发育树的星形拓扑结构一起，将这些基因组部分确定为重组热点。这些发现进一步支持肠病毒基因组片段独立进化的概念，并表明需要更先进的分型方法。一系列可用的系统发育方法也进行了比较，以有效地检测重组肠道病毒。
The complete nucleotide sequences of eight Human enterovirus B (HEV-B) strains were determined, representing five serotypes, E6, E7, El 1, CVB3 and CVB5, which were isolated in the former Soviet Union between 1998 and 2002. All strains were mosaic recombinants and only the VP2-VP3-VP1 genome region was similar to that of the corresponding prototype HEV-B strains. In seven of the eight strains studied, the 2C-3D genome region was most similar to the prototype E30, EV74 and EV75 strains, whilst the remaining strain was most similar to the prototype El and E9 strains in the non-structural protein genome region. Most viruses also bore marks of additional recombination events in this part of the genome. In the 5' non-translated region, all strains were more similar to the prototype E9 than to other enteroviruses. In most cases, recombination mapped to the VP4 and 2ABC genome regions. This, together with the star-like topology of the phylogenetic trees for these genome regions, identified these genome parts as recombination hot spots. These findings further support the concept of independent evolution of enterovirus; genome fragments and indicate a requirement for more advanced typing approaches. A range of available phylogenetic methods was also compared for efficient detection of recombination in enteroviruses.