Meiocyte-Specific and AtSPO11-1-Dependent Small RNAs and Their Association with Meiotic Gene Expression and Recombination
Meiocyte-Specific and AtSPO11-1-Dependent Small RNAs and Their Association with Meiotic Gene Expression and Recombination
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减数分裂细胞特异性和 AtSPO11-1 依赖性小 RNA 及其与减数分裂基因表达和重组的关联。
DOI：
10.1105/tpc.18.00511
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发表时间：
2019-02-01
期刊：
影响因子：
11.6
通讯作者：
Wang, Yingxiang
中科院分区：
文献类型：
--
作者：
Huang, Jiyue;Wang, Cong;Wang, Yingxiang
减数分裂重组确保染色体精确分离，并导致有性繁殖真核生物的遗传多样性。在过去的几十年里，减数分裂重组的遗传调控在许多生物中得到了广泛的研究。然而，内源性减数分裂特异性小RNA（ms-sRNA; 21-24核苷酸[nt]）的作用及其参与减数分裂重组的作用尚不清楚。在这里，我们对野生型拟南芥（Arabidopsis thaliana）和spo 11 -1（一种双链断裂形成缺陷的突变体）的性母细胞和叶片的总小RNA（sRNA）和信使RNA群体进行了测序，我们发现了2，409个ms-sRNA簇，其中1，660个是孢子化11-1（AtSPO 11 -1）依赖的。与有丝分裂小干扰RNA富集在基因间区域并与基因沉默相关不同，ms-sRNA显著富集在基因区域，并以与DNA甲基化无关的方式与在性母细胞中优先表达的基因（即拟南芥SKP 1-LIKE 1和RAD 51）呈现正相关。我们还发现AtSPO 11 -1依赖的sRNA与ms-sRNA相比具有不同的特征，并且倾向于与两种已知类型的减数分裂重组热点基序（即CTT重复和富含A的基序）相关。这些结果揭示了不同的减数分裂和有丝分裂sRNA景观，并提供了新的见解sRNA如何与基因表达的性母细胞和减数分裂重组。
Meiotic recombination ensures accurate chromosome segregation and results in genetic diversity in sexually reproducing eukaryotes. Over the last few decades, the genetic regulation of meiotic recombination has been extensively studied in many organisms. However, the role of endogenous meiocyte-specific small RNAs (ms-sRNAs; 21-24 nucleotide [nt]) and their involvement in meiotic recombination are unclear. Here, we sequenced the total small RNA (sRNA) and messenger RNA populations from meiocytes and leaves of wild type Arabidopsis (Arabidopsis thaliana) and meiocytes of spo11-1, a mutant defective in double-strand break formation, and we discovered 2,409 ms-sRNA clusters, 1,660 of which areSPORULATION 11-1 (AtSPO11-1)-dependent. Unlike mitotic small interfering RNAs that are enriched in intergenic regions and associated with gene silencing, ms-sRNAs are significantly enriched in genic regions and exhibit a positive correlation with genes that are preferentially expressed in meiocytes (i.e. Arabidopsis SKP1-LIKE1 and RAD51), in a fashion unrelated to DNA methylation. We also found that AtSPO11-1-dependent sRNAs have distinct characteristics compared with ms-sRNAs and tend to be associated with two known types of meiotic recombination hotspot motifs (i.e. CTT-repeat and A-rich motifs). These results reveal different meiotic and mitotic sRNA landscapes and provide new insights into how sRNAs relate to gene expression in meiocytes and meiotic recombination.