Generation of a mouse for conditional excision of progesterone receptor
Generation of a mouse for conditional excision of progesterone receptor
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DOI：
10.1002/dvg.20227
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发表时间：
2006-08-01
期刊：
影响因子：
1.5
通讯作者：
Iruela-Arispel, M. Luisa
中科院分区：
文献类型：
--
作者：
Hashimoto-Partyka, Minako K.;Lydon, John P.;Iruela-Arispel, M. Luisa
孕激素受体（PR）在哺乳动物雌性生殖的几个方面都是必需的。PR缺失小鼠存在重叠缺陷，这妨碍了对其在排卵、妊娠、乳腺生物学和性行为中的多种功能的理解。我们产生了一个PR条件切除（PRCE）等位基因，其中loxP位点位于外显子1的侧面。纯合子的PRCE雌性具有生育能力，看起来功能正常。使用Ella-cre小鼠，cre介导的全基因组外显子1的切除导致外显子1和PR蛋白的系统性丢失。与PR基因敲除（PRKO）的雌性小鼠一样，该零等位基因纯合子的雌性小鼠不育。条件性PR缺失将有助于研究PR在正常发育和疾病中的时空作用。
The progesterone receptor (PR) is required for several aspects of mammalian female reproduction. PR null mice have overlapping defects that preclude an understanding of its multiple functions in ovulation, pregnancy, mammary gland biology, and sexual behavior. We have generated a PR conditional excision (PRCE) allele in which loxP sites flank exon 1. Homozygous PRCE females are fertile and appear to be functionally normal. Global cre mediated excision of the floxed exon 1 using Ella-cre mice resulted in systemic loss of exon 1 and PR protein. Female mice homozygous for this null allele were sterile, as expected for PR knockout (PRKO) females. Conditional loss of PR will facilitate investigation of the spatial and temporal roles of PR in both normal development and disease.