COLORIMETRIC METHOD FOR THE DETERMINATION OF ALIPHATIC AMINES IN THE PRESENCE OF AMMONIA

COLORIMETRIC METHOD FOR THE DETERMINATION OF ALIPHATIC AMINES IN THE PRESENCE OF AMMONIA
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DOI:
10.1042/bj0650128
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发表时间:
1957-01-01
影响因子:
4.1
通讯作者:
KING, HK
KING, HK
中科院分区:
生物学3区
文献类型:
--
作者:
EKLADIUS, L;KING, HK

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本文介绍了生物样品中低级脂肪胺的微量测定方法。将胺在碱性pH下蒸汽蒸馏,并使其与1-氯-2,4-二硝基苯反应。黄色产物是通过化学计量法估算的。氨不干扰测定。
A method is described for the microdetermlnation of lower aliphatic amines in biological material. The amine is steam-distilled at alkaline pH and allowed to react with 1-chloro-2 4-dinitrobenzene. The yellow product is estimated spectrophotometrically. Ammonia does not interfere with the determination.