Sox9b/sox9a2-EGFP transgenic medaka reveals the morphological reorganization of the gonads and a common precursor of both the female and male supporting cells
Sox9b/sox9a2-EGFP transgenic medaka reveals the morphological reorganization of the gonads and a common precursor of both the female and male supporting cells
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DOI：
10.1002/mrd.20764
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发表时间：
2008-03-01
影响因子：
2.5
通讯作者：
Tanaka, Minoru
中科院分区：
文献类型：
--
作者：
Nakamura, Shuhei;Aoki, Yumiko;Tanaka, Minoru
我们建立了一个模拟sox9b/sox9a2表达的增强型绿色荧光蛋白(EGFP)转基因青竹品系，以分析这种鱼性腺的形态重组和性腺形成过程中表达sox9b的细胞的特征。生殖细胞迁移到性腺区后，发现单个性腺原基中的一簇表达EGFP的细胞被体细胞沿喙尾轴分开，形成双侧叶。我们在这些转基因鱼中观察到，绿色荧光蛋白的表达只在生殖细胞直接周围的体细胞中持续存在。随着性别分化的进行，dmrt1和FOXL2分别开始在XY和XX性腺的EGFP表达细胞中表达。这表明，表达sox9b的细胞重组为性腺的两个叶，然后分化为支持细胞或颗粒细胞，作为支持细胞的共同前体。因此，我们的sox9b-EGFP青竹系统将在未来性腺发育的研究中发挥作用。
We have established an enhanced green fluorescent protein (EGFP) transgenic medaka line that mimics the expression of sox9b/sox9a2 to analyze the morphological reorganization of the gonads and characterize the sox9b-expressing cells during gonadal formation in this fish. After the germ cells have migrated into the gonadal areas, a cluster of EGFP-expressing cells in the single gonadal primordium was found to be separated by the somatic cells along the rostrocaudal axis and form the bilateral lobes. We observed in these transgenic fish that EGFP expression persists only in the somatic cells directly surrounding the germ cells. As sex differentiation proceeds, dmrt1 and foxl2 begin to be expressed in the EGFP-expressing cells in the XY and the XX gonads, respectively. This indicates that the sox9b-expressing cells reorganize into two lobes of the gonad and then differentiate into Sertoli or granulosa cells, as common precursors of the supporting cells. Hence, our sox9b-EGFP medaka system will be useful in future studies of gonadal development.