Insulin-like peptides (AmILP1 and AmILP2) differentially affect female caste development in the honey bee (Apis mellifera L.)
Insulin-like peptides (AmILP1 and AmILP2) differentially affect female caste development in the honey bee (Apis mellifera L.)
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DOI：
10.1242/jeb.085779
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发表时间：
2013-12-01
影响因子：
2.8
通讯作者：
Amdam, Gro V.
中科院分区：
文献类型：
--
作者：
Wang, Ying;Azevedo, Sergio V.;Amdam, Gro V.
蜜蜂雌性幼虫接受的食物决定了她是否发育成大型长寿的可育蜂王或短命的不育工蜂。通过后生动物中成熟的营养感知和生长促进功能，胰岛素/胰岛素样生长因子 1 信号通路 (IIS) 已成为研究食物环境差异如何转化为负责蜂王工蜂决定的内部信号的研究焦点。然而，蜜蜂幼虫中两种胰岛素受体 (AmInR) 的低表达水平以及一种 AmInR 未能影响种姓分化，与 IIS 在工蜂发育中的经典生长促进作用存在潜在冲突。鉴于这种明显的矛盾，以及这两个AmInR的结合伴侣和亲和力尚未确定的事实，我们通过使用双链RNA（dsRNA）介导的基因敲低方法对蜜蜂幼虫中的胰岛素样肽基因（AmILP1和AmILP2）进行了功能研究。我们发现 AmILP1 dsRNA 治疗降低了保幼激素 (JH) 水平，而 AmILP2 敲低则导致卵巢大小减小。血糖滴度并未受到治疗的显着影响。从这些结果中，我们得出结论，AmILP2 转录物水平可能会影响特定器官的发育，例如卵巢和体重，而种姓分化的更一般特征，例如下颌骨，可能需要额外的调节剂。此外，JH 的产生可能受到大脑局部表达的 AmILP1 的调节，类似于果蝇中某些 ILP 的功能。
The food a honey bee female larva receives determines whether she develops into a large long-lived fertile queen or a short-lived sterile worker. Through well-established nutrient-sensing and growth-promoting functions in metazoans, the insulin/insulin-like growth factor 1 signaling (IIS) pathway has become a focal topic in investigations on how differences in food environment can be translated into internal signals responsible for queen-worker determination. However, low expression levels of two insulin receptors (AmInRs) in honey bee larvae and the failure of one AmInR to influence caste differentiation are in potential conflict with such a classical growth-promoting role of IIS in queen-worker development. In view of such an apparent contradiction, and the fact that binding partners and affinities of these two AmInRs have not been worked out, we performed a functional study on insulin-like peptide genes (AmILP1 and AmILP2) in honey bee larvae by using a double-stranded RNA (dsRNA)-mediated gene knockdown approach. We found that juvenile hormone (JH) levels were diminished by AmILP1 dsRNA treatment, while the AmILP2 knockdown caused a reduction in ovary size. Blood sugar titers were not significantly affected by the treatments. From these results we conclude that AmILP2 transcript levels may influence specific organ development, such as the ovary and body mass, while more general traits of caste differentiation, such as mandibles, may require additional regulators. In addition, JH production may be regulated by AmILP1 expressed locally in the brain, similar to the function of certain ILPs in Drosophila.