Simultaneous in vivo optical quantification of key metabolic and vascular endpoints reveals tumor metabolic diversity in murine breast tumor models.
Simultaneous in vivo optical quantification of key metabolic and vascular endpoints reveals tumor metabolic diversity in murine breast tumor models.
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DOI：
10.1002/jbio.201800372
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发表时间：
2019-04
影响因子：
2.8
通讯作者：
Ramanujam N
中科院分区：
文献类型：
--
作者：
Zhu C;Li M;Vincent T;Martin HL;Crouch BT;Martinez AF;Madonna MC;Palmer GM;Dewhirst MW;Ramanujam N
治疗性地利用血管和代谢终点对于转化癌症研究变得至关重要，因为改变的血管分布和失调的代谢是两个重要的癌症标志。利用一种新开发的定量光谱系统，在体内研究了三种具有不同转移潜能的乳腺肿瘤细胞系的代谢和血管表型。与正常组织相比，所有肿瘤细胞系具有不同的代谢和血管特征，并且对于转移性（4T1）肿瘤，但对于微转移性（4T07）和非转移性（67NR）肿瘤，代谢（葡萄糖摄取和线粒体膜电位）和血管（氧饱和度和血红蛋白浓度）参数之间存在强正相关性。一项纵向研究表明，4T1肿瘤的血管和代谢终点均增加至特定肿瘤大小阈值，超过该阈值，这些参数均降低。代谢和血管参数之间的同步变化以及这些终点之间的强正相关性沿着表明，除了糖酵解之外，4T1肿瘤还依赖于强氧化磷酸化。这项研究说明了我们的光学技术的巨大潜力，提供有价值的动态信息之间的相互作用的代谢和血管状态的肿瘤，具有重要意义的转化癌症调查
Therapeutically exploiting vascular and metabolic endpoints becomes critical to translational cancer studies because altered vascularity and deregulated metabolism are two important cancer hallmarks. The metabolic and vascular phenotypes of three sibling breast tumor lines with different metastatic potential are investigated in vivo with a newly developed quantitative spectroscopy system. All tumor lines have different metabolic and vascular characteristics compared to normal tissues, and there are strong positive correlations between metabolic (glucose uptake and mitochondrial membrane potential) and vascular (oxygen saturations and hemoglobin concentrations) parameters for metastatic (4T1) tumors but not for micrometastatic (4T07) and nonmetastatic (67NR) tumors. A longitudinal study shows that both vascular and metabolic endpoints of 4T1 tumors increased up to a specific tumor size threshold beyond which these parameters decreased. The synchronous changes between metabolic and vascular parameters, along with the strong positive correlations between these endpoints suggest that 4T1 tumors rely on strong oxidative phosphorylation in addition to glycolysis. This study illustrates the great potential of our optical technique to provide valuable dynamic information about the interplay between the metabolic and vascular status of tumors, with important implications for translational cancer investigations
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影响因子：
2.2
作者：
Jayaraman, S
通讯作者：
Jayaraman, S
影响因子：
4.8
作者：
Lu, Xin;Bennet, Bryson;Kang, Yibin
通讯作者：
Kang, Yibin
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作者：
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影响因子：
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作者：
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