Development of tandem mass spectrometry-based creatinine measurement using dried blood spot for newborn mass screening
Development of tandem mass spectrometry-based creatinine measurement using dried blood spot for newborn mass screening
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DOI：
10.1038/pr.2017.56
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发表时间：
2017-08-01
影响因子：
3.6
通讯作者：
Saitoh, Shinji
中科院分区：
文献类型：
--
作者：
Nakano, Masaru;Uemura, Osamu;Saitoh, Shinji
背景：先天性肾脏和尿路异常（CAKUT）是儿童慢性肾脏病（CKD）最常见的病因。然而，尚未开发出强有力的大规模筛查方法来检测 CAKUT 患者，这使得早期干预以预防进行性肾衰竭具有挑战性。 方法：我们应用串联质谱 (MS/MS) 来测量干血斑 (DBS) 分析中的肌酐 (Cr) 值，该值已用于新生儿大规模筛查。随后，我们评估了 CKD 儿科患者通过 MS/MS 测量的 DBS Cr 与通过常规方法测量的血清 Cr 之间的相关性。最后，在 190 名足月健康新生儿出生后第 4-6 天测量了 DBS Cr。 结果：我们建立了一个基于 MS/MS 的 DBS Cr 测量系统。儿科患者的 DBS Cr 测量结果显示与血清 Cr 有很强的相关性（r=0.86；P
BACKGROUND: Congenital anomalies of the kidney and urinary tract (CAKUT) are the most prevalent etiologies of pediatric chronic kidney disease (CKD). However, no robust mass screening methods have been developed to detect patients with CAKUT, making early intervention to prevent progressive renal failure challenging.METHODS: We applied tandem mass spectrometry (MS/MS) to measure the creatinine (Cr) value from dried blood spot (DBS) analysis, which has been used for newborn mass screening. Subsequently, we evaluated the correlation between DBS Cr measured by MS/MS and serum Cr measured by the conventional method in pediatric patients with CKD. Finally, DBS Cr was measured in 190 full-term, healthy newborns on days 4-6 after birth.RESULTS: We established a system of MS/MS-based measurement of Cr from DBS. Measured DBS Cr in the pediatric patients showed a strong association with serum Cr (r=0.86; P