High-throughput multiplexed fluorescence-activated droplet sorting
High-throughput multiplexed fluorescence-activated droplet sorting
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DOI：
10.1038/s41378-018-0033-2
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发表时间：
2018-10-22
影响因子：
7.9
通讯作者：
Taly, Valerie
中科院分区：
文献类型：
--
作者：
Caen, Ouriel;Schutz, Simon;Taly, Valerie
荧光激活液滴分选（FADS）是基于液滴的微流体技术提供的最重要的特征之一。然而，迄今为止，它不允许与流式细胞术提供的高通量多重分选能力竞争。在这里，我们展示了使用基于分散的FADS，允许同时对多达五个不同的液滴群体进行分类。我们的系统提供了在单个实验运行中选择不同表型的液滴以分离初始异质群体的方法。我们的实验结果合理化的帮助下，在电场中的液滴的驱动的数值模型提供指导方针的预测排序设计的放大或缩小的微系统。
Fluorescence-activated droplet sorting (FADS) is one of the most important features provided by droplet-based microfluidics. However, to date, it does not allow to compete with the high-throughput multiplexed sorting capabilities offered by flow cytometery. Here, we demonstrate the use of a dielectrophoretic-based FADS, allowing to sort up to five different droplet populations simultaneously. Our system provides means to select droplets of different phenotypes in a single experimental run to separate initially heterogeneous populations. Our experimental results are rationalized with the help of a numerical model of the actuation of droplets in electric fields providing guidelines for the prediction of sorting designs for upscaled or downscaled microsystems.