Hierarchical metabolomics demonstrates substantial compositional similarity between genetically modified and conventional potato crops
Hierarchical metabolomics demonstrates substantial compositional similarity between genetically modified and conventional potato crops
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DOI：
10.1073/pnas.0503955102
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发表时间：
2005-10-04
影响因子：
11.1
通讯作者：
Draper, J
中科院分区：
文献类型：
--
作者：
Catchpole, GS;Beckmann, M;Draper, J
转基因植物是否可能在总体代谢物组成中包含意想不到的、潜在的不良变化，目前存在争议。然而，需要开发适当的分析技术和可接受的成分相似性指标。我们描述了一个全面的比较，在现场种植的转基因和传统的马铃薯块茎的总代谢物，使用分层的方法开始与快速代谢组学“指纹”，以指导更详细的分析代谢物的显着差异被怀疑。这一策略的核心是数据分析程序，能够从复杂的代谢组数据中生成经验证的、可重复的比较指标。我们表明，除了有针对性的变化，这些转基因马铃薯在这项研究中似乎基本上等同于传统品种。
There is current debate whether genetically modified (GM) plants might contain unexpected, potentially undesirable changes in overall metabolite composition. However, appropriate analytical technology and acceptable metrics of compositional similarity require development. We describe a comprehensive comparison of total metabolites in field-grown GM and conventional potato tubers using a hierarchical approach initiating with rapid metabolome "fingerprinting" to guide more detailed profiling of metabolites where significant differences are suspected. Central to this strategy are data analysis procedures able to generate validated, reproducible metrics of comparison from complex metabolome data. We show that, apart from targeted changes, these GM potatoes in this study appear substantially equivalent to traditional cultivars.