Virus-Induced Gene Silencing in Wheat and Related Monocot Species.
Virus-Induced Gene Silencing in Wheat and Related Monocot Species.
复制标题
小麦和相关单子叶植物物种中病毒诱导的基因沉默。
DOI：
10.1007/978-1-0716-1875-2_6
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Panwar V
中科院分区：
文献类型：
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作者：
Panwar V
基因组测序计划和结构基因组学的进展正在产生与特定农艺性状相关的植物中的大量候选基因。因此，需要快速和高通量的功能基因组学方法来验证这些基因的生物学功能，特别是对于除了少数模式植物物种之外的农艺学上重要的作物。这可以通过利用可用的基因敲除或转基因方法来实现，但这些方法可能需要相当多的时间和精力，特别是在具有大而复杂的基因组的作物如小麦中。因此，任何加速基因功能验证的工具都是特别有益的，特别是在遗传上难以转化的谷类作物植物中。一种这样的反向遗传学工具是病毒诱导的基因沉默（VIGS），其依赖于植物的天然抗病毒RNA沉默防御机制。VIGS用于以瞬时方式下调靶基因表达，其持续足够长的时间以确定其对特定性状的影响。基于大麦条纹花叶病毒（Barley stripe mosaic virus，BSMV）的VIGS技术是一种快速、高效、廉价的基因功能分析工具。在这里，我们提出了详细的协议BSMV介导的VIGS强大的基因沉默在面包小麦和相关物种。
Advances made in genome sequencing projects and structural genomics are generating large repertoire of candidate genes in plants associated with specific agronomic traits. Rapid and high-throughput functional genomics approaches are therefore needed to validate the biological function of these genes especially for agronomically important crops beyond the few model plant species. This can be achieved by utilizing available gene knockout or transgenic methodologies, but these can take considerable time and effort particularly in crops with large and complex genomes such as wheat. Therefore, any tool that expedites the validation of gene function is of particular benefit especially in cereal crop plants that are genetically difficult to transform. One such reverse genetics tool is virus-induced gene silencing (VIGS) which relies on the plants’ natural antiviral RNA silencing defence mechanism. VIGS is used to downregulate target gene expression in a transient manner which persists long enough to determine its effect on a specific trait. VIGS based onBarley stripe mosaic virus(BSMV) is rapid, powerful, efficient, and relatively inexpensive tool for the analysis of gene function in cereal species. Here we present detailed protocols for BSMV-mediated VIGS for robust gene silencing in bread wheat and related species.