Cell orientation by a microgrooved substrate can be predicted by automatic control theory
Cell orientation by a microgrooved substrate can be predicted by automatic control theory
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DOI：
10.1529/biophysj.105.067967
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发表时间：
2006-06-01
影响因子：
3.4
通讯作者：
Gruler, Hans
中科院分区：
文献类型：
--
作者：
Kemkemer, Ralf;Jungbauer, Simon;Gruler, Hans
细胞有能力测量和响应细胞外信号，如化学分子和地形表面特征，通过改变他们的方向。在这里，我们检查了暴露于沟槽地形的培养的人类黑素细胞的取向。为了预测细胞的方向响应，我们用一个自动控制器模型来描述细胞的行为。通过考虑系统（单元1衬底）的对称性，获得了细胞响应对凹槽高度和空间频率的预测依赖性。一个基本的结果是，自动控制器响应槽高度和空间频率的乘积的平方或对对称槽的长径比。实验验证了这一理论预测，在实验中，黑素细胞暴露于微制造的聚（二甲基硅氧烷）底物中，这些底物具有平行的矩形凹槽，高度(h)在25至200 nm之间，空间频率(L)在100至500 mm（-1）之间。此外，对元胞自动控制器的模型进行了扩展，考虑了不同导频信号同时作用的情况。
Cells have the ability to measure and respond to extracellular signals like chemical molecules and topographical surface features by changing their orientation. Here, we examined the orientation of cultured human melanocytes exposed to grooved topographies. To predict the cells' orientation response, we describe the cell behavior with an automatic controller model. The predicted dependence of the cell response to height and spatial frequency of the grooves is obtained by considering the symmetry of the system (cell 1 substrate). One basic result is that the automatic controller responds to the square of the product of groove height and spatial frequency or to the aspect ratio for symmetric grooves. This theoretical prediction was verified by the experiments, in which melanocytes were exposed to microfabricated poly( dimethylsiloxane) substrates having parallel rectangular grooves of heights (h) between 25 and 200 nm and spatial frequencies (L) between 100 and 500 mm(-1). In addition, the model of the cellular automatic controller is extended to include the case of different guiding signals acting simultaneously.