A large scale analysis of protein-protein interactions in the nitrogen-fixing bacterium Mesorhizobium loti.
A large scale analysis of protein-protein interactions in the nitrogen-fixing bacterium Mesorhizobium loti.
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DOI：
10.1093/dnares/dsm028
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发表时间：
2008-02-29
期刊：
影响因子：
--
通讯作者：
Sato S
中科院分区：
文献类型：
--
作者：
Shimoda Y;Shinpo S;Kohara M;Nakamura Y;Tabata S;Sato S
蛋白质-蛋白质相互作用（PPI）的全局视图是一种有用的方式来分配生物学角色的大量蛋白质的全基因组序列预测。在这里，我们系统地分析了PPIs的固氮土壤细菌Mesorhizobium loti使用改良的高通量酵母双杂交系统。本研究的主要目的是为M.与共生固氮相关且在其他根瘤菌物种中保守的loti蛋白，特别是具有调节功能的蛋白和未注释的蛋白。通过筛选1542个基因作为诱饵，3121个独立的相互作用，涉及1804个蛋白质（占总蛋白质编码基因的24%）被确定，每个相互作用进行了评估，使用相互作用的一般性（IG）措施和一般功能的相互作用的合作伙伴。本研究中检测到的大多数PPI是新的相互作用，揭示了共生固氮和信号转导基因之间的潜在功能关系。此外，我们还通过未注释蛋白与已知蛋白的相互作用预测了它们的推定功能。本文的结果代表了对M. loti和提供有用的实验线索，阐明根瘤菌基因的生物学功能，不能直接分配从他们的基因组序列。
Global viewing of protein–protein interactions (PPIs) is a useful way to assign biological roles to large numbers of proteins predicted by complete genome sequence. Here, we systematically analyzed PPIs in the nitrogen-fixing soil bacterium Mesorhizobium loti using a modified high-throughput yeast two-hybrid system. The aims of this study are primarily on the providing functional clues to M. loti proteins that are relevant to symbiotic nitrogen fixation and conserved in other rhizobium species, especially proteins with regulatory functions and unannotated proteins. By the screening of 1542 genes as bait, 3121 independent interactions involving 1804 proteins (24% of the total protein coding genes) were identified and each interaction was evaluated using an interaction generality (IG) measure and the general features of the interacting partners. Most PPIs detected in this study are novel interactions revealing potential functional relationships between genes for symbiotic nitrogen fixation and signal transduction. Furthermore, we have predicted the putative functions of unannotated proteins through their interactions with known proteins. The results described here represent new insight into protein network of M. loti and provide useful experimental clues to elucidate the biological function of rhizobial genes that can not be assigned directly from their genomic sequence.
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影响因子：
4.1
作者：
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通讯作者：
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64.8
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3.2
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通讯作者：
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