Ovarian histopathology of bitches immunized with porcine zonae pellucidae.
Ovarian histopathology of bitches immunized with porcine zonae pellucidae.
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用猪透明带免疫的母犬的卵巢组织病理学。
DOI：
10.1111/j.1600-0897.1988.tb00242.x
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Palumbo,N
中科院分区：
文献类型：
--
作者：
Mahi-Brown,CA;Yanagimachi,R;Nelson,ML;Yanagimachi,H;Palumbo,N
用粗制或部分纯化的猪透明带蛋白免疫母犬，观察卵巢组织病理学变化，以确定发情周期异常的原因。大多数免疫母犬有卵巢细胞。用cPZP免疫的那些具有衬有薄层颗粒细胞的卵泡囊肿，而在用pPZP免疫的那些中，囊肿衬有基底膜和一团黄素化细胞。在用cPZP免疫的两只母犬中，卵母细胞仅存在于原始卵泡中。在用人血清白蛋白免疫的母犬或12只未处理的母犬中未发现类似的异常。从交配的免疫母犬输卵管中冲洗出来的卵母细胞正在退化，这可能是这类母犬不育的主要原因。免疫后2-6周研究的卵巢显示卵母细胞和颗粒细胞之间的缝隙连接通讯没有丢失，也没有观察到任何炎症反应。IgG结合到卵巢的冷冻切片的荧光素蛋白A染色所显示的卵巢。PZP免疫母犬的异常发情周期似乎是由卵泡发育不全或囊肿形成引起的，但这些疾病的病因尚不清楚。
The ovarian histopathology of bitches immunized with crude (cPZP) or partially purified (pPZP) porcine zona pellucida proteins was examined in order to determine the cause of abnormal estrous cycles. The majority of immunized bitches had ovarian cytes. Those immunized with cPZP had follicular cysts lined with a thin layer of granulosa cells, while in those immunized with pPZP, the cysts were lined by a basement membrane with a clump of luteinized cells. In two bitches immunized with cPZP, oocytes were present only in primordial follicles. Similar abnormalities were not found in a bitch immunized with human serum albumin or in 12 untreated bitches. Oocytes flushed from the oviducts of mated, immunized bitches were degenerating, which may have been a primary cause of infertility in such bitches. Ovaries studied 2–6 weeks after immunization showed no loss of gap junctional communication between oocytes and granulosa cells, nor was any inflammatory reaction seen. IgG was bound to the zona as revealed by fluoresceinated protein A staining of frozen sections of those ovaries. Abnormal estrous cycles in PZP‐immunized bitches appear to result from follicular dysgenesis or cyst formation, but the etiology of these conditions is unresolved.