MS/MS with high detection efficiency and mass resolving power for product ions in Fourier transform ion cyclotron resonance mass spectrometry.
MS/MS with high detection efficiency and mass resolving power for product ions in Fourier transform ion cyclotron resonance mass spectrometry.
复制标题
MS/MS 对傅里叶变换离子回旋共振质谱中的产物离子具有高检测效率和质量分辨能力。
DOI：
10.1021/ac00080a024
复制
发表时间：
1994
影响因子：
7.4
通讯作者：
Wahl,MC
中科院分区：
文献类型：
--
作者：
Guan,S;Marshall,AG;Wahl,MC
本文提出了一种新的FT-ICR MS/MS捕获离子分析方法。在双阱FT-ICR/MS仪器的源阱中分离的母离子受到非共振偶极激发以促进碰撞诱导解离（CID），而产物离子在氩碰撞气体存在下同时受到宽带方位角四极激发轴向化。以这种方式，产物离子的径向扩散损失大大减少;此外，轴向化的产物离子然后可以有效地转移到分析器阱以进行高分辨率检测。对于环肽短杆菌肽S的CID产物离子，证明了FT-ICR检测效率、质量分辨率（m/z< 1000时m/Am> 20 000）和质量准确度（对于m/z< 1000的肽片段< 50 ppm）的显著改善。
We present a new FT-ICR method for MS/MS of trapped ions. Parent ions isolated in the source trap of a dual-trap FT-ICR/MS instrument are subjected to off-resonance dipolar excitation to promote collision-induced dissociation (CID), while product ions are simultaneously axialized by broad-band azimuthal quadrupolar excitation in thepresence of argon collision gas. In this way, radial diffusional loss of product ions is greatly reduced; moreover, the axialized product ions may then be efficiently transferred to the analyzertrap for high-resolution detection. Significant improvements in FT-ICR detection efficiency, mass resolving power (m/Am> 20 000 at m/z< 1000), and mass accuracy (< 50 ppm for peptide fragments of m/z< 1000) are demonstrated for CID product ions of the cyclic peptide, gramicidin S.