CCN5 Expression in mammals. II. Adult rodent tissues
CCN5 Expression in mammals. II. Adult rodent tissues
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DOI：
10.1007/s12079-007-0013-z
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发表时间：
2007-09-01
影响因子：
4.1
通讯作者：
Castellot, John J., Jr.
中科院分区：
文献类型：
--
作者：
Gray, Mark R.;Malmquist, Jennifer A.;Castellot, John J., Jr.
CCN 5是一种分泌的肝素和雌激素调节的基质细胞蛋白，抑制脊椎动物平滑肌细胞增殖和运动。CCN 5在大多数器官和组织中的整个鼠胚胎发育中表达。然而，在胚胎发育完成后，我们假设CCN 5的分布将主要限于一小部分组织，包括动脉、子宫、气道和消化道的平滑肌细胞。由于CCN 5在体外抑制平滑肌细胞的增殖，因此它可能具有防止体内过度生长的功能。相反，CCN家族的另一个成员CCN 2在体外促进平滑肌细胞增殖，因此预期其表达水平在未损伤的正常成人组织中将是低的。使用抗CCN 5和抗CCN 2抗体对来自成人组织和器官的冷冻切片进行化学分析。如预期，在动脉、子宫、细支气管和消化道中检测到这两种蛋白，并且在许多其他组织中也检测到这两种蛋白，包括胰腺、脾脏、肝脏、骨骼肌、卵巢、睾丸、胸腺、脑、嗅上皮和肾脏。CCN 5和CCN 2蛋白存在于平滑肌、内皮细胞、上皮细胞、骨骼肌、神经系统细胞和许多其他细胞类型中。在许多细胞中，CCN 5和CCN 2都存在于细胞核中。CCN 5和CCN 2通常具有相似的表达位点，而不是具有相反的定位模式。CCN 5和CCN 2的广泛分布表明，这两种蛋白质具有超出先前在特定细胞和病理模型中鉴定的那些生物学功能的额外生物学功能。
CCN5 is a secreted heparin-and estrogen-regulated matricellular protein that inhibits vertebrate smooth muscle cell proliferation and motility. CCN5 is expressed throughout murine embryonic development in most organs and tissues. However, after embryonic development is complete, we hypothesized that CCN5 distribution would be largely restricted to small set of tissues, including smooth muscle cells of the arteries, uterus, airway, and digestive tract. Because CCN5 inhibits proliferation of smooth muscle cells in vitro, it might function to prevent excessive growth in vivo. In contrast, another member of the CCN family, CCN2, promotes smooth muscle cell proliferation in vitro, and thus it was expected that its expression levels would be low in uninjured normal adult tissues. Frozen sections from adult tissues and organs were analyzed immunohistochemically using anti-CCN5 and anti-CCN2 antibodies. Both proteins were detected in arteries, the uterus, bronchioles, and the digestive tract as expected, and also in many other tissues including the pancreas, spleen, liver, skeletal muscle, ovary, testis, thymus, brain, olfactory epithelium, and kidney. CCN5 and CCN2 protein was found in smooth muscle, endothelial cells, epithelial cells, skeletal muscle, cells of the nervous system, and numerous other cell types. In many cells, both CCN5 and CCN2 was present in the nucleus. Rather than having opposite patterns of localization, CCN5 and CCN2 often had similar sites of expression. The wide distribution of both CCN5 and CCN2 suggests that both proteins have additional biological functions beyond those previously identified in specific cellular and pathological models.