Diffusion of bacteriophages through artificial biofilm models
Diffusion of bacteriophages through artificial biofilm models
复制标题
噬菌体通过人工生物膜模型的扩散
DOI：
10.1002/btpr.742
复制
发表时间：
2012
影响因子：
2.9
通讯作者：
Jun Hu
中科院分区：
文献类型：
--
作者：
Y. Negishi;M. Mizuno;M. Hirayama;M. Omatoi;T. Takayama;A. Iwase;and A. Kudo;Jun Hu
The simple two‐chamber diffusion method was improved to study the diffusion properties of bacteriophage (phage) T4 through a model biofilm agarose gel membrane (AGM) embedded with dead hostEscherichia coliK12 cells. The apparent diffusion coefficient (Dapp) of phage T4 was calculated to be 2.4 × 10−12m2/s in 0.5% AGM, which was lower than the coefficient of 4.2 × 10−12m2/s in 0.5% AGM without host cells. The phage adsorption process by dead host cells slowed the apparent phage diffusion. The Langmuir adsorption equation was used to simulate phage adsorption under different multiplicity of infections (MOIs); the maximum adsorbed phage MOI was calculated to be 417 PFU/CFU, and the Langmuir adsorption constantKLwas 6.9 × 10−4CFU/PFU. To evaluate the effects of phage proliferation on diffusion, a simple syringe‐based biofilm model was developed. The phage was added into this homogenous biofilm model when the host cells were in an exponential growth phase, and the apparent diffusion coefficient was greatly enhanced. We concluded thatDappof phages through biofilms could be distinctly affected by phage adsorption and proliferation, and that the idea ofDappand these methods can be used to study diffusion properties through real biofilms. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2012
