Identification of a second Escherichia coli groE gene whose product is necessary for bacteriophage morphogenesis.
Identification of a second Escherichia coli groE gene whose product is necessary for bacteriophage morphogenesis.
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DOI：
10.1073/pnas.78.3.1629
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发表时间：
1981-03
影响因子：
11.1
通讯作者：
K. Tilly;H. Murialdo;C. Georgopoulos
中科院分区：
文献类型：
--
作者：
K. Tilly;H. Murialdo;C. Georgopoulos
先前的工作已经发现了大肠杆菌基因座groE的存在，groE对于细菌生长、λ噬菌体和T4噬菌体头部形态发生以及T5噬菌体尾部组装是必不可少的。我们对λ groE+转导的突变体及其缺失和点突变衍生物的遗传和生化分析表明，groE基因座由两个紧密连锁的基因组成。一个groE基因groEL已显示编码65，000 Mr多肽的合成，而第二个groES编码15，000 Mr多肽的合成。大约一半的groE-细菌分离株属于groES互补组。就λ噬菌体头部形态发生和非允许温度下的细菌生长而言，任一基因中的GroE突变都会导致相似的表型。
Previous work has uncovered the existence of an Escherichia coli locus, groE, that is essential for bacterial growth, lambda phage and T4 phage head morphogenesis, and T5 phage tail assembly. Our genetic and biochemical analyses of lambda groE+ transducing phages and their deletion and point mutant derivatives show that the groE locus consists of two closely linked genes. One groE gene, groEL, has been shown to encode the synthesis of a 65,000 Mr polypeptide, whereas the second, groES, codes for the synthesis of a 15,000 Mr polypeptide. About half of the groE- bacterial isolates fall into the groES complementation group. GroE mutations in either gene cause similar phenotypes, with respect to lambda phage head morphogenesis and bacterial growth at nonpermissive temperatures.