Suppression of the SUGAR-DEPENDENT1 triacylglycerol lipase family during seed development enhances oil yield in oilseed rape (Brassica napus L.)
Suppression of the SUGAR-DEPENDENT1 triacylglycerol lipase family during seed development enhances oil yield in oilseed rape (Brassica napus L.)
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DOI：
10.1111/pbi.12021
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发表时间：
2013-04-01
影响因子：
13.8
通讯作者：
Eastmond, Peter J.
中科院分区：
文献类型：
--
作者：
Kelly, Amelie A.;Shaw, Eve;Eastmond, Peter J.
提高油籽作物的生产力是植物育种家和生物技术学家面临的一项重要挑战。迄今为止，通过代谢途径工程提高种子油产量的尝试主要集中在提高合成能力上。然而，在许多生物体的组织中，众所周知，油含量是由合成代谢和分解代谢决定的。事实上，据报道，随着种子干燥，油菜籽 (Brassica napus L.) 的含油量在发育的最后阶段会下降约 10%。在这里，我们表明，在种子发育过程中对 SUGAR-DEPENDENT1 三酰甘油脂肪酶基因家族进行 RNAi 抑制，可以使温室中的种子、植物或单位面积的油产量增加高达 8%，而对种子活力的不利影响非常小。因此，抑制脂肪分解可能成为提高油料作物油产量的新方法。
Increasing the productivity of oilseed crops is an important challenge for plant breeders and biotechnologists. To date, attempts to increase oil production in seeds via metabolic pathway engineering have focused on boosting synthetic capacity. However, in the tissues of many organisms, it is well established that oil levels are determined by both anabolism and catabolism. Indeed, the oil content of rapeseed (Brassica napus L.) has been reported to decline by approximately 10% in the final stage of development, as the seeds desiccate. Here, we show that RNAi suppression of the SUGAR-DEPENDENT1 triacylglycerol lipase gene family during seed development results in up to an 8% gain in oil yield on either a seed, plant or unit area basis in the greenhouse, with very little adverse impact on seed vigour. Suppression of lipolysis could therefore constitute a new method for enhancing oil yield in oilseed crops.