MOLECULAR CONSTRUCTION OF CLOSTRIDIUM-BOTULINUM TYPE-C PROGENITOR TOXIN AND ITS GENE ORGANIZATION
MOLECULAR CONSTRUCTION OF CLOSTRIDIUM-BOTULINUM TYPE-C PROGENITOR TOXIN AND ITS GENE ORGANIZATION
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DOI：
10.1006/bbrc.1994.2805
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发表时间：
1994-12-15
影响因子：
3.1
通讯作者：
OGUMA, K
中科院分区：
文献类型：
--
作者：
FUJINAGA, Y;INOUE, K;OGUMA, K
C型肉毒梭菌的16 S祖毒素通过将神经毒素与称为无毒-非HA和血凝素（HA）的无毒组分缀合而制成。发现HA由具有53、33、22类似于23和17 kDa分子量的亚组分组成。由于我们先前确定了神经毒素、无毒-非HA和HA-33基因的全核苷酸序列，因此对其余HA亚组分的基因进行了克隆和核苷酸测序。鉴定了编码16.7 kDa（HA-17）和70.6 kDa蛋白的两个开放阅读框。HA-53和HA-22与23相似的N-末端氨基酸序列表明，70.6 kDa蛋白质被分裂成53个，22个与23 kDa蛋白质相似的蛋白质改变翻译，22个与23 kDa蛋白质相似的蛋白质由至少四种分子量略有不同的蛋白质组成。(C)1994年出版社出版。
The 16S progenitor toxin of Clostridium botulinum type C is made up by conjugation of a neurotoxin with nontoxic components designated as nontoxic-nonHA and hemagglutinin (HA). The HA was found to be composed of subcomponents having 53, 33, 22 similar to 23, and 17 kDa molecular masses. Since we previously determined the whole nucleotide sequences of the genes for neurotoxin, nontoxic-nonHA, and HA-33, the cloning and nucleotide sequencing of the genes for the remaining HA subcomponents were performed. Two open reading frames coding for 16.7 kDa (HA-17) and 70.6 kDa proteins were identified. The N-terminal amino acid sequences of HA-53 and HA-22 similar to 23 indicated that the 70.6 kDa protein is split into 53 and the 22 similar to 23 kDa proteins alter translation and that the 22 similar to 23 kDa protein consists of at least four proteins showing slightly different molecular weights. (C) 1994 Academic Press, Inc.