Ingestion of heat-treated Lactobacillus rhamnosus GG prevents development of atopic dermatitis in NC/Nga mice
Ingestion of heat-treated Lactobacillus rhamnosus GG prevents development of atopic dermatitis in NC/Nga mice
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DOI：
10.1111/j.1365-2222.2006.02645.x
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发表时间：
2007-02-01
影响因子：
6.1
通讯作者：
Matsuda, H.
中科院分区：
文献类型：
--
作者：
Sawada, J.;Morita, H.;Matsuda, H.
背景连续口服活的鼠李糖乳杆菌GG（L. GG）对患有特应性皮炎的妊娠受试者及其子女，抑制特应性皮炎的频率。然而，这种抑制作用所涉及的机制和免疫系统的细节仍然是推测性的。方法采用NC/Nga小鼠作为异位性皮炎的动物模型，以含或不含热处理L. GG在怀孕和哺乳期间，断奶后。结果控制NC/Nga小鼠提出的空气不受控制的条件下自发表现出典型的皮肤病变非常相似的特应性皮炎患者。另一方面，含热处理L. GG抑制特应性皮肤病变的发生和发展，伴随着受影响的皮肤部位的肥大细胞和嗜酸性粒细胞数量减少。用L.与对照小鼠相比，GG显示血浆IL-10水平显着增加，而饲喂L.的小鼠之间脾脏CD 4（+）CD 25（+）调节性T细胞比例无显着差异。GG和对照小鼠。IL-10 mRNA在Peyer集合淋巴结和肠系膜淋巴结中的表达增强。结论热处理后的枸杞子中的某些成分可能与热处理后的枸杞子中的某些成分有关。GG可能具有延迟特应性皮炎的发作并抑制其发展的能力，这可能是通过在肠淋巴器官和全身水平中强烈诱导IL-10。
Background Continuous oral administration of live Lactobacillus rhamnosus GG (L. GG) to pregnant subjects with atopic dermatitis and their children, suppressed the frequency of atopic dermatitis. The details of mechanisms and immune systems involved in this suppressive effect, however, remain speculative.Objective We sought to clarify suppressive mechanisms of L. GG on atopic dermatitis by using NC/Nga mice, a model of human atopic dermatitis.Methods Maternal mice and infant mice were fed with food containing or not containing heat-treated L. GG during pregnancy and breastfeeding, and after weaning.Results Control NC/Nga mice raised under an air-uncontrolled condition spontaneously manifested typical skin lesions very similar to those in patients with atopic dermatitis. On the other hand, administration of food containing heat-treated L. GG inhibited the onset and development of atopic skin lesions, accompanied by smaller numbers of mast cells and eosinophils in the affected skin sites. Mice fed with L. GG showed a significant increase in plasma IL-10 levels compared with control mice, while there was no significant difference in the proportion of splenic CD4(+)CD25(+) regulatory T cells between mice fed with L. GG and control mice. The IL-10 mRNA expression was enhanced in both Peyer's patches and mesenteric lymph nodes in mice fed with L. GG.Conclusion These findings suggest that some components of heat-treated L. GG may have an ability to delay the onset and suppress the development of atopic dermatitis, probably through a strong induction of IL-10 in intestinal lymphoid organs and systemic levels.