Tyrosyl and cyclic AMP-dependent protein kinase activities in BHK cells that express viral pp60src.
Tyrosyl and cyclic AMP-dependent protein kinase activities in BHK cells that express viral pp60src.
复制标题
表达病毒 pp60src 的 BHK 细胞中酪氨酰和环 AMP 依赖性蛋白激酶活性。
DOI：
10.1128/mcb.4.5.973-977.1984
复制
发表时间：
1984
影响因子：
5.3
通讯作者：
RoskoskiJr,R
中科院分区：
文献类型：
--
作者：
Clinton,GM;RoskoskiJr,R
Protein kinase activities were measured in Rous sarcoma virus-infected baby hamster kidney (BHK) cells that express v-src(BHK [v-src]) and compared with those of revertant and control BHK cells. We observed about a fivefold-higher tyrosine phosphorylating activity in BHK (v-src) cell extracts, which was due tosrcbut not other cellular tyrosyl kinase activities since preincubation with anti-srcserum reduced the activity to control cell levels. The cyclic AMP-dependent protein kinase activity was also altered when v-srcwas expressed. Resolution of the two cyclic AMP-dependent isozymes from the detergent-soluble fraction of cells revealed that the type I activity was selectively decreased about fivefold in BHK (v-src) cells.
