Complete genome sequence and analysis of Wolinella succinogenes
Complete genome sequence and analysis of Wolinella succinogenes
复制标题
DOI：
10.1073/pnas.1932838100
复制
发表时间：
2003-09-30
影响因子：
11.1
通讯作者：
Schuster, SC
中科院分区：
文献类型：
--
作者：
Baar, C;Eppinger, M;Schuster, SC
要了解致病细菌的起源和出现，了解其非致病近缘细菌的遗传库存是先决条件。因此，确定了与致病菌幽门螺杆菌和空肠弯曲菌密切相关的Wolinella succinogenes的2.11兆碱基基因组序列。尽管被认为对其牛宿主无致病性，但 W. succinogenes 拥有大量与已知细菌毒力因子同源的基因。其中许多基因是通过横向基因转移获得的，因为发现部分毒力质粒 pVir 和 Winked 糖基化基因簇在空肠弯曲菌和 W succinogenes 基因组岛之间是同线性的。与其他适应宿主的细菌相比，W succinogenes 确实拥有迄今为止在任何细菌基因组中发现的最高密度的细菌传感器激酶，以及 GGDEF 蛋白家族的复杂信号传导电路。由于对 W succinogenes 基因组的分析还揭示了与土壤和植物相关细菌相关的基因，例如 nif 基因，因此 W succinogenes 可能代表了在宿主之外具有生命周期的ε变形菌的成员。
To understand the origin and emergence of pathogenic bacteria, knowledge of the genetic inventory from their nonpathogenic relatives is a prerequisite. Therefore, the 2.11-megabase genome sequence of Wolinella succinogenes, which is closely related to the pathogenic bacteria Helicobacter pylori and Campylobacter jejuni, was determined. Despite being considered nonpathogenic to its bovine host, W. succinogenes holds an extensive repertoire of genes homologous to known bacterial virulence factors. Many of these genes have been acquired by lateral gene transfer, because part of the virulence plasmid pVir and an Winked glycosylation gene cluster were found to be syntenic between C jejuni and genomic islands of W succinogenes. In contrast to other host-adapted bacteria, W succinogenes does harbor the highest density of bacterial sensor kinases found in any bacterial genome to date, together with an elaborate signaling circuitry of the GGDEF family of proteins. Because the analysis of the W succinogenes genome also revealed genes related to soil- and plant-associated bacteria such as the nif genes, W succinogenes may represent a member of the epsilon proteobacteria with a life cycle outside its host.