TRANSCRIPTION AND DNA-SEQUENCE OF THE BAMHI L-FRAGMENT OF B95-8 EPSTEIN-BARR VIRUS
TRANSCRIPTION AND DNA-SEQUENCE OF THE BAMHI L-FRAGMENT OF B95-8 EPSTEIN-BARR VIRUS
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DOI：
10.1002/j.1460-2075.1984.tb01933.x
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发表时间：
1984-01-01
期刊：
影响因子：
11.4
通讯作者：
BARRELL, BG
中科院分区：
文献类型：
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作者：
BIGGIN, M;FARRELL, PJ;BARRELL, BG
测定了B 95 -8 EB病毒（EBV）DNA的BamHI L片段的序列。使用S1作图和体外转录或引物延伸技术，将5个启动子的转录起始点映射到该片段。用12-O-十四烷酰佛波醇-13-乙酸酯（TPA）处理绒猴白细胞B 95 -8细胞后，由这些启动子转录的细胞质poly（A）+RNA水平显著增加。对于这些启动子中的3个，添加膦酰基乙酸（PAA）抑制TPA的作用，表明它们引起晚期裂解周期RNA。另外两个启动子产生早期RNA。北方印迹分析表明，1个晚期启动子启动2个转录本，其大小差异是由于不同的剪接模式。这2种RNA编码EBV的350/300和250/200千道尔顿包膜糖蛋白。这些蛋白质的序列将用于生产预防EBV感染的合成疫苗。
The sequence of the BamHI L fragment of B95-8 Epstein-Barr virus (EBV) DNA was determined. The transcription starts of 5 promoters were mapped to this fragment, using S1 mapping and either in vitro transcription or the primer extension technique. Dramatically increased levels of cytoplasmic poly(A)+ RNAs, transcribed from these promoters, occur after treatment of marmoset leukocyte B95-8 cells with 12-O-tetradecanoylphorbol-13-acetate (TPA). For 3 of these promoters addition of phosphonoacetic acid (PAA) inhibits the effect of TPA, indicating that they give rise to late lytic cycle RNA. The other 2 promoters give rise to early RNA. Northern blot analysis indicates that 1 of the late promoters initiates 2 transcripts whose size differences are due to different splicing patterns. These 2 RNA code for the 350/300 and 250/200 kilodalton envelope glycoproteins of EBV. The sequences of these proteins would be of use in the production of a synthetic vaccine to prevent EBV infection.