Actin and myosin inhibitors block elongation of kinetochore fibre stubs in metaphase crane-fly spermatocytes
Actin and myosin inhibitors block elongation of kinetochore fibre stubs in metaphase crane-fly spermatocytes
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DOI：
10.1007/s00709-007-0265-8
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发表时间：
2007-12-01
期刊：
影响因子：
2.9
通讯作者：
Pickett-Heaps, J. D.
中科院分区：
文献类型：
--
作者：
Forer, A.;Spurck, T.;Pickett-Heaps, J. D.
我们用紫外线微束切割鹤-蝇中期精母细胞的单个动粒纺锤体纤维。然后，我们跟踪了“动粒残根”的生长，动粒纤维的残留物仍然附着在动粒上。动粒存根通过在动粒上添加微管蛋白亚基以恒定的速度伸长，因此伸长与动粒微管中的微管微管中的微管流量有关。肌动蛋白抑制剂细胞松弛素D和乳杆菌凝集素A以及肌球蛋白抑制剂丁二酮单肟能阻断短桩的伸长。我们得出结论，肌动蛋白和肌球蛋白参与了动粒微管的伸长过程，从而产生了微管蛋白流量。我们认为，肌动蛋白和肌球蛋白与纺锤体基质协同作用，推动着丝粒纤维向极方向移动，从而在未受照射的细胞中引起残根延长和后期染色体移动。
We used an ultraviolet microbeam to cut individual kinetochore spindle fibres in metaphase crane-fly spermatocytes. We then followed the growth of the "kinetochore stubs", the remnants of kinetochore fibres that remain attached to kinetochores. Kinetochore stubs elongate with constant velocity by adding tubulin subunits at the kinetochore, and thus elongation is related to tubulin flux in the kinetochore microtubules. Stub elongation was blocked by cytochalasin D and latrunculin A, actin inhibitors, and by butanedione monoxime, a myosin inhibitor. We conclude that actin and myosin are involved in generating elongation and thus in producing tubulin flux in kinetochore microtubules. We suggest that actin and myosin act in concert with a spindle matrix to propel kinetochore fibres poleward, thereby causing stub elongation and generating anaphase chromosome movement in nonirradiated cells.