Intrinsic susceptibility to radiation-induced apoptosis of human lymphocyte subpopulations
Intrinsic susceptibility to radiation-induced apoptosis of human lymphocyte subpopulations
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DOI：
10.1016/s0360-3016(03)00637-0
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发表时间：
2003-11-01
影响因子：
7
通讯作者：
Thomas, G
中科院分区：
文献类型：
--
作者：
Schmitz, A;Bayer, J;Thomas, G
目的：为了评估内在的放射敏感性，确定淋巴细胞亚群对放射诱导的细胞凋亡的敏感性。研究的参数包括测量可靠性、表型方差、个体内和个体间的变异性，以及辐射诱导的细胞凋亡和自发性细胞凋亡之间的相关性。 方法和材料：63 名健康志愿者的静止淋巴细胞在一年内采样四次，并在体外进行伽马射线照射。使用 6 色流式细胞术测量 T4、T8 和 B 淋巴细胞亚群的后续凋亡（膜联蛋白 V）。测量自发性凋亡，并根据剂量效应曲线量化放射敏感性。结果：解冻和短期培养后，三个淋巴细胞亚群的自发性凋亡和辐射诱导的细胞凋亡（放射敏感性）均不同，T4 抵抗力最强，B 最敏感。自发性细胞凋亡和辐射诱导的细胞凋亡在所有细胞类型中都是相关的，并且个体之间的差异明显高于个体内部的差异。随着年龄的增长，T8 的自发性细胞凋亡和辐射诱导的细胞凋亡均出现小幅但高度显着的增加，但 T4 和 B 则不然。事实证明，T8 和 B 的放射敏感性与性别无关，而女性 T4 淋巴细胞的放射敏感性低于男性。 T4和T8放射敏感性松散相关，且两者均与B放射敏感性无关。结论：淋巴细胞亚群自发性和辐射诱导的细胞凋亡的倾向在个体之间存在差异。此外，根据细胞类型，年龄和性别是影响这些参数的因素。 (C) 2003 爱思唯尔公司。
Purpose: With the aim to evaluate intrinsic radiosensitivity, the susceptibility of lymphocyte subpopulations to radiation-induced apoptosis was determined. The investigated parameters included measurement reliability, phenotypic variance, intra- and inter-individual variability, and correlations between radiation-induced and spontaneous apoptosis.Methods and Materials: Quiescent lymphocytes of 63 healthy volunteers, sampled up to four times over a 1-year period were gamma-irradiated in vitro. Subsequent apoptosis (annexin V) was measured for T4-, T8-, and B-lymphocyte subpopulations using 6-color flow cytometry. Spontaneous apoptosis was measured and radiosensitivity was quantified from the dose-effect curves.Results: After thawing and short-term culture, both spontaneous apoptosis as well as radiation-induced apoptosis (radiosensitivity) differed among the three lymphocyte subpopulations, with T4 being most resistant, and B most sensitive. Spontaneous and radiation-induced apoptosis were correlated in all cell types, and variance between individuals was considerably higher than variance within individuals for both. A small but highly significant increase of both spontaneous and radiation-induced apoptosis was observed with age for T8, but not for T4 and B. Radiosensitivity of T8 and B proved to be sex-independent, whereas female T4 lymphocytes were less radiosensitive than those from males. T4 and T8 radiosensitivities were loosely correlated, and neither of them was related to B radiosensitivity.Conclusions: Tendency to spontaneous and radiation-induced apoptosis of lymphocyte subpopulations differs among individuals. In addition, depending on the cell types, age and sex are factors influencing these parameters. (C) 2003 Elsevier Inc.