Secretion of phytase from the roots of several plant species under phosphorus-deficient conditions
Secretion of phytase from the roots of several plant species under phosphorus-deficient conditions
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DOI：
10.1023/a:1004264002524
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发表时间：
1997-08-01
期刊：
影响因子：
4.9
通讯作者：
Tadano, T
中科院分区：
文献类型：
--
作者：
Li, MG;Osaki, M;Tadano, T
缺磷增加了不同植物根部植酸酶的分泌。用透析膜管收集16种植物根部的分泌型植酸酶，在营养液中磷供应不足或充足的情况下生长。在低磷处理下，所有植物的植酸酶和酸性磷酸酶的活性都随着低磷处理的增加而增加。植株在缺磷条件下根系植酸酶分泌量以斜纹臂尾轮藻CIAT606、柱花草CIAT184和番茄最高，其次是布氏臂尾轮虫、柱花草CIAT6780、柱花草CIAT2950、紫花苜蓿、白三叶草和果园草，最低的是鬼针草CIAT621、柱花草CIAT621、柱花草CIAT 10280、旱稻、蒂莫西、红顶、三叶草和白三叶。在7个供试物种中，可检测到一条与小麦麸皮植酸酶多克隆抗体反应的免疫反应蛋白条带，其相对分子质量为35-40 kD。这些结果表明，分泌型植酸酶可能为某些植物利用土壤中的六磷酸肌醇提供了一种有效的机制。
Phosphorus (P) deficiency increased the secretion of phytases from roots of various plant species. The secretory phytases were collected with a dialysis membrane tube for 24 hours from roots of sixteen plant species grown with low or adequate supply of P in nutrient solutions. The activity of not only secretory phytase, but also acid phosphatase, increased with the low P treatment in all of the plant species examined. Secretion of phytase by the roots under P-deficient conditions was highest in Brachiaria decumbens CIAT 606, Stylosanthes guianensis CIAT 184 and tomato, moderate in Brachiaria brizantha CIAT6780, Stylosanthes guianensis CIAT 2950, alfalfa, white clover and orchard grass, and lowest in Andropgon gayanus CIAT 621, Stylosanthes capitata CIAT 10280, upland rice, timothy, redtop, alsike clover, red clover and white lupin plants. An immunoreactive protein band that reacted with a polyclonal antibody raised against wheat bran phytase, corresponding to molecular weight 35-40 kD, could be detected in seven of the species tested. These results indicate that the secretory phytase may provide an efficient mechanism for certain plants to utilize inositol hexaphosphate in soil.