IL-1 plays a critical role in oral, put not dermal, wound healing
IL-1 plays a critical role in oral, put not dermal, wound healing
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DOI：
10.4049/jimmunol.167.9.5316
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发表时间：
2001-11-01
影响因子：
4.4
通讯作者：
Amar, S
中科院分区：
文献类型：
--
作者：
Graves, DT;Nooh, N;Amar, S
伤口愈合是一个精心策划的复杂过程，导致受损组织的修复。损伤后，促炎细胞因子作为炎症过程的重要调节剂。IL-1表达被认为是愈合所必需的;然而，其作用也与延迟的伤口修复有关。目前，没有共识或直接证据表明IL-1活性在愈合过程中发挥核心作用。本研究旨在确定IL-1 R信号转导在小鼠腭部或头皮切除伤口愈合结果中的作用，与匹配的野生型小鼠相比，IL-1 R 1（-/-）有针对性的缺失。进行组织形态学分析，以评估愈合程度和多形核细胞和单核吞噬细胞的募集。14天后，野生型小鼠的口内伤口完全闭合，而IL-1 R1（-/-）动物仅部分闭合（50%）。在IL-1 R1（-/-）小鼠中，愈合组织表现出持续的炎性细胞浸润，这在野生型动物中没有发生。用抗生素治疗显著减少了实验动物的持续性炎症浸润并改善了愈合。与口腔伤口相反，在IL-1 R1（-/-）和野生型小鼠中，头皮伤口中多形核细胞的跳动和募集速率相似。本数据强调了IL-1在具有挑战性的环境中伤口愈合中的重要性，并确定了其通过保护开放性伤口免受细菌侵害而促进愈合过程的主要作用。在挑战性较低的环境中，新结缔组织的产生及其被迁移上皮覆盖的情况受IL-1活性缺乏的影响最小。
Wound healing is a well-orchestrated complex process leading to the repair of injured tissues. After injury, proinflammatory cytokines act as important modulators of the inflammatory process. IL-1 expression has been regarded as necessary for healing; however, its effects have also been implicated in delayed wound repair. Currently, there is no consensus or direct evidence that IL-1 activity plays a central role in the healing process. The present investigation was undertaken to define the role of IL-1R signaling in the healing outcome of an excisional wound in the palate or scalp of mice that had targeted deletions of the IL-1R type 1 (IL-1R1(-/-)) compared with matched wild-type mice. Histomorphometric analysis was undertaken to assess the degree of healing and the recruitment of polymorphonuclear and mononuclear phagocytes. After 14 days, wild-type mice exhibited complete closure of intraoral wounds, while IL-1R1(-/-) animals had only partial closure (50%). In the IL-1R1(-/-) mice, healing tissues exhibited a persistent inflammatory cell infiltrate, which did not occur in wild-type animals. Treatment with antibiotics significantly diminished the persistent inflammatory infiltrate and improved healing in the experimental animals. In contrast to oral wounds, the rate of beating and recruitment of polymorphonuclear cells in scalp wounds was similar in IL-1R1(-/-) and wild-type mice. The present data underscore the importance of IL-1 in wound healing in a challenging environment and identify its principal role in facilitating the healing process by protecting an open wound from bacterial insult. In a less challenging environment, the production of new connective tissue and its coverage by migrating epithelium are minimally affected by the absence of IL-1 activity.