Quantification of microheterogeneity in glioblastoma multiforme with ex vivo high-resolution magic-angle spinning (HRMAS) proton magnetic resonance spectroscopy
Quantification of microheterogeneity in glioblastoma multiforme with ex vivo high-resolution magic-angle spinning (HRMAS) proton magnetic resonance spectroscopy
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DOI：
10.1093/neuonc/2.2.87
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发表时间：
2000-04-01
期刊：
影响因子：
15.9
通讯作者：
Gonzalez, RG
中科院分区：
文献类型：
--
作者：
Cheng, LL;Anthony, DC;Gonzalez, RG
显微异质性是脑肿瘤病理学中常规观察到的神经病理学特征，尽管常规组织学技术很容易证明显微异质性，但这些技术仅在活检或手术时测量肿瘤状态，并不提示肿瘤可能的进展。根据病理标准校准的生化筛查技术将极大地帮助根据其生物学活性预测肿瘤的进展。在这里，我们首次展示了高分辨率魔角旋转(HRMAS)质子磁共振波谱(H-1MRS)，这是1997年引入的一项技术，可以在相同的完整组织标本中产生分辨率良好的细胞代谢物光谱，同时显示组织的组织病理学特征。因此，对于同一手术标本，观察到的生化变化和肿瘤组织病理学特征可以相互关联，从而避免了由肿瘤微观异质性引起的问题。我们分析了一位44岁患者手术切除的单个人类多形性胶质母细胞瘤的多个标本。每个标本首先用HRMAS H-1MRS测定肿瘤代谢物，然后用定量组织病理学评估，用HRMAS测量的乳酸和流动脂浓度线性反映肿瘤坏死率，而且磷胆碱/胆碱的代谢比率与高度细胞恶性胶质瘤的百分比线性相关，HRMAS H-1MRS量化肿瘤代谢变化，结合后续的组织病理学，有可能加深我们对肿瘤发育过程中异质性的生物化学的了解，从而最终提高我们诊断、鉴定和评估肿瘤进展的准确性。
Microheterogeneity is a routinely observed neuropathologic characteristic in brain tumor pathology, Although microheterogeneity is readily documented by routine histologic techniques, these techniques only measure tumor status at the time of biopsy or surgery and do not indicate likely tumor progression. A biochemical screening technique calibrated against pathologic standards would greatly assist in predicting tumor progression from its biological activity. Here we demonstrate for the first time that proton magnetic resonance spectroscopy (H-1 MRS) with high-resolution magic-angle spinning (HRMAS), a technique introduced in 1997, can presence tissue histopathologic features while producing well-resolved spectra of cellular metabolites in the identical intact tissue specimens. Observed biochemical alterations and tumor histopathologic characteristics can thus be correlated for the same surgical specimen, obviating the problems caused by tumor microheterogeneity. We analyzed multiple specimens of a single human glioblastoma multiforme surgically removed from a 44-year-old patient. Each specimen was first measured with HRMAS H-1 MRS to determine tumor metabolites, then evaluated by quantitative histopathology, The concentrations of lactate and mobile lipids measured with HRMAS linearly reflected the percentage of tumor necrosis, Moreover, metabolic ratios of phosphorylcholine to choline correlated linearly with the percentage of the highly cellular malignant glioma, The quantification of tumor metabolic changes with HRMAS H-1 MRS, in conjunction with subsequent histopathology of the same tumor specimen, has the potential to further our knowledge of the biochemistry of tumor heterogeneity during development, and thus ultimately to improve our accuracy in diagnosing, characterizing, and evaluating tumor progression.