Silibinin is a potent sensitizer of UVA radiation-induced oxidative stress and apoptosis in human keratinocyte HaCaT cells.
Silibinin is a potent sensitizer of UVA radiation-induced oxidative stress and apoptosis in human keratinocyte HaCaT cells.
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DOI：
10.1111/j.1751-1097.2011.01050.x
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发表时间：
2012-09
影响因子：
3.3
通讯作者：
Agarwal R
中科院分区：
文献类型：
--
作者：
Narayanapillai S;Agarwal C;Tilley C;Agarwal R
Ultraviolet A radiation (UVA, 315–400nm), which constitutes approximately 95% of the UV irradiation in natural sunlight reaching earth surface, is a major environmental risk factor associated with human skin cancer pathogenesis. UVA is an oxidizing agent that causes significant damage to cellular components through the release of reactive oxygen species (ROS) and leads to photoaging and photocarcinogenesis. Here we investigate the effect of silibinin, the flavonolignan from Silybum marianum, on UVA-induced ROS and cell death in human keratinocyte cell line HaCaT. In addition, the effect of silibinin on UVA-induced intracellular ROS-mediated endoplasmic reticulum (ER) stress was also analyzed. UVA irradiation resulted in ROS production and apoptosis in HaCaT cells in a dose-dependent manner, and the ROS levels and apoptotic index were found to be elevated significantly when the cells were treated with 75μM silibinin for 2h before UVA exposure. When the cells were pretreated with 10mM N-acetyl cysteine (NAC), the enhancement of UVA-induced apoptosis by silibinin was compromised. Furthermore, we found that silibinin enhances ER stress-mediated apoptosis in HaCaT cells by increasing the expression of CHOP protein. These results suggest that silibinin may be beneficial in the removal of UVA-damaged cells and the prevention of skin cancer.
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影响因子：
3.4
作者：
Dou, M;Han, YT;Hou, YY
通讯作者：
Hou, YY
DOI：
10.1016/s1011-1344(00)00006-3
发表时间：
2000-02-01
影响因子：
5.4
作者：
Parisi, AV;Wong, JCF
通讯作者：
Wong, JCF
DOI：
10.1159/000064535
发表时间：
2002-09-01
期刊：
SKIN PHARMACOLOGY AND APPLIED SKIN PHYSIOLOGY
影响因子：
--
作者：
de Gruijl, FR
通讯作者：
de Gruijl, FR
影响因子：
4.7
作者：
Dhanalakshmi, S;Mallikarjuna, GU;Agarwal, R
通讯作者：
Agarwal, R
影响因子：
6.5
作者：
Anand, S;Chakrabarti, E;Maytin, EV
通讯作者：
Maytin, EV