ISOLATION AND CHARACTERIZATION OF MICROSATELLITE MARKERS FOR DIPTERONIA DYERANA (SAPINDACEAE), AN ENDANGERED ENDEMIC SPECIES IN CHINA
ISOLATION AND CHARACTERIZATION OF MICROSATELLITE MARKERS FOR DIPTERONIA DYERANA (SAPINDACEAE), AN ENDANGERED ENDEMIC SPECIES IN CHINA
复制标题
中国濒危特有物种双翅目（SAPINDACEAE）微卫星标记的分离和鉴定
DOI：
10.3732/ajb.1100185
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发表时间：
2011-10-01
影响因子：
3
通讯作者：
Qiu, Ying-Xiong
中科院分区：
文献类型：
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作者：
Chen, Chen;Ren, Bing-Bing;Qiu, Ying-Xiong
研究前提：为中国濒危特有种双翅目双翅目建立微卫星标记，以研究该物种的群体遗传学。方法与结果：采用AFLP快速分离重复序列（FIASCO）方案，对文山县和蒙自县两个种群的31个个体进行测试，成功扩增出12个微卫星标记，显示出多态性。总体而言，每个位点的等位基因数量在 7 至 25 之间，平均为 12.3。其中9个标记能够在中华双翅目中扩增。结论：这些微卫星标记应有助于进一步研究双翅目种群遗传学和繁殖生态学。
Premise of the study : Microsatellite markers were developed for Dipteronia dyerana, an endangered endemic species in China, to study the population genetics of this species.Methods and Results : Using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol, 12 microsatellite markers that were successfully amplified showed polymorphism when tested on 31 individuals from two populations in the counties of Wenshan and Mengzi. Overall, the number of alleles per locus ranged between seven and 25, with an average of 12.3. Nine of these markers were able to be amplified in D. sinensis.Conclusions : These microsatellite markers should facilitate further studies on the population genetics and the reproductive ecology of Dipteronia